The DNA‐binding protein of Pf1 filamentous bacteriophage: amino‐acid sequence and structure of the gene
The DNA‐binding protein of Pf1 filamentous bacteriophage: amino‐acid sequence and structure of the gene
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Pf1丝状噬菌体的DNA结合蛋白：氨基酸序列和基因结构
DOI：
10.1002/j.1460-2075.1982.tb01156.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
G. B. Petersen
中科院分区：
文献类型：
--
作者：
K. Maéda;G. Kneale;A. Tsugita;Nicholas J. Short;R. Perham;D. Hill;G. B. Petersen
噬菌体Pf1单链DNA结合蛋白的氨基酸序列和相应基因的核苷酸序列已被确定。该蛋白质有144个氨基酸，分子量为15400；该基因由435个核苷酸组成。通过 Edman 降解、羧肽酶 A、B 和 P 消化完整蛋白质以及胰凝乳蛋白酶、金黄色葡萄球菌 V8 蛋白酶和胰蛋白酶消化衍生的肽来确定氨基酸序列。将 Pf1 DNA 片段随机克隆到 M13 中后，通过双脱氧法测定核苷酸序列。铜绿假单胞菌特异性噬菌体 Pf1 和大肠杆菌噬菌体 fd 的 DNA 结合蛋白的氨基酸序列之间无法建立序列同源性。
The amino‐acid sequence of the single‐stranded DNA‐binding protein of bacteriophage Pf1 and the nucleotide sequence of the corresponding gene have been determined. The protein has 144 amino acids and a molecular weight of 15 400; the gene consists of 435 nucleotides. The amino‐acid sequence was determined by Edman degradation, carboxypeptidase A, B, and P digestion of intact protein and of peptides derived by chymotrypsin, Staphylococcus aureus V8 protease, and trypsin digestion. The nucleotide sequence was determined by the dideoxy method after random cloning of fragments of Pf1 DNA into M13. No sequence homology could be established between the amino‐acid sequence of the DNA‐binding protein of Pseudomonas aeruginosa‐specific bacteriophage Pf1 and bacteriophage fd of Escherichia coli.