Rapid detection and enumeration of Legionella pneumophila in hot water systems by solid-phase cytometry
Rapid detection and enumeration of Legionella pneumophila in hot water systems by solid-phase cytometry
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DOI：
10.1128/aem.70.3.1651-1657.2004
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发表时间：
2004-03-01
影响因子：
4.4
通讯作者：
Lebaron, P
中科院分区：
文献类型：
--
作者：
Aurell, H;Catala, P;Lebaron, P
建立了一种快速灵敏检测热水系统中嗜肺军团菌的新方法。该方法是基于IF检测结合固相细胞术检测。本方法可在3 ~ 4个月内对自来水样品中嗜肺乳杆菌血清1、嗜肺乳杆菌血清2 ~ 6、8 ~ 10、12 ~ 15进行计数。该方法的灵敏度在每升10至100个细菌之间，主要受膜过滤能力的限制。对15株非军团菌进行特异性检测，未发现交叉反应。当该方法应用于自然水体时，将嗜肺乳杆菌的直接计数与标准培养法获得的CFU数量进行比较。直接计数始终高于培养计数，两种方法的比值为1.4 ~ 325。固相细胞术为热水系统中嗜肺乳杆菌的筛选提供了一种快速、灵敏的替代培养方法。
A new method for the rapid and sensitive detection of Legionella pneumophild in hot water systems has been developed. The method is based on an IF assay combined with detection by solid-phase cytometry. This method allowed the enumeration of L. pneumophila serogroup 1 and L. pneumophila serogroups 2 to 6, 8 to 10, and 12 to 15 in tap water samples within 3 to 4 It. The sensitivity of the method was between 10 and 100 bacteria per liter and was principally limited by the filtration capacity of membranes. The specificity of the antibody was evaluated against 15 non-Legionella strains, and no cross-reactivity was observed. When the method was applied to natural waters, direct counts of L. pneumophila were compared with the number of CFU obtained by the standard culture method. Direct counts were always higher than culturable counts, and the ratio between the two methods ranged from 1.4 to 325. Solid-phase cytometry offers a fast and sensitive alternative to the culture method for L. pneumophila screening in hot water systems.