An unusual pattern of spontaneous mutations recovered in the halophilic archaeon Haloferax volcanii
An unusual pattern of spontaneous mutations recovered in the halophilic archaeon Haloferax volcanii
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DOI：
10.1534/genetics.106.069666
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发表时间：
2007-05-01
期刊：
影响因子：
3.3
通讯作者：
Grogan, Dennis W.
中科院分区：
文献类型：
--
作者：
Mackwan, Reena R.;Carver, Geraldine T.;Grogan, Dennis W.
嗜盐古菌Haloferax volcanii的乳清酸：磷酸核糖转移酶（pyrE 2）基因的自发突变被5-氟乳清酸加尿嘧啶选择，在波动和空分数测试中的速率类似于2 × 10（-8）/细胞分裂，但在突变积累测试中的速率类似于6 × 10（-8）/细胞分裂。相应的基因组突变率大大低于其他嗜温微生物DNA基因组的基础上类似的靶基因观察到的。突变谱以插入/缺失3 bp的Multiples为主。导致这种不寻常突变模式的生物体特性可能包括由高染色体拷贝数引起的表型滞后和由短直接重复序列引起的链错配的有效促进。
Spontaneous mutations in the orotate:phosphoribosyl transferase (pyrE2) gene of the halophilic archaeon Haloferax volcanii were selected by 5-fluoroorotic acid plus uracil at a rate of similar to 2 X 10(-8)/cell division in fluctuation and null-fraction tests but similar to 6 X 10(-8)/cell division in mutation-accumulation tests. The corresponding genomic mutation rates were substantially lower than those observed for other mesophilic microbial DNA genomes on the basis of similar target genes. The mutational spectrum was dominated by indels adding or deleting Multiples of 3 bp. Properties of the organism contributing to this unusual mutational pattern may include phenotypic lag caused by a high chromosomal copy number and efficient promotion of strand misalignments by short direct repeats.