Stalled Hox promoters as chromosomal boundaries
Stalled Hox promoters as chromosomal boundaries
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DOI：
10.1101/gad.1807309
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发表时间：
2009-07-01
影响因子：
10.5
通讯作者：
Levine, Michael
中科院分区：
文献类型：
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作者：
Chopra, Vivek S.;Cande, Jessica;Levine, Michael
许多发育控制基因在早期果蝇胚胎中含有停滞的RNA聚合酶II（Pol II），包括八个Hox基因中的四个。在这里，我们提出的证据表明，停滞的Hox启动子具有内在的绝缘体活性。这些启动子的增强子阻断活性依赖于抑制Pol II延伸的一般转录因子，包括DSIF和NELF复合物的组分。常规绝缘体的活动也受损的胚胎含有降低水平的DSIF和NELF。因此，启动子近端失速因子可能有助于促进绝缘子-启动子相互作用。我们认为，停滞的启动子有助于组织染色体环域内的基因复合体。
Many developmental control genes contain stalled RNA Polymerase II (Pol II) in the early Drosophila embryo, including four of the eight Hox genes. Here, we present evidence that the stalled Hox promoters possess an intrinsic insulator activity. The enhancer-blocking activities of these promoters are dependent on general transcription factors that inhibit Pol II elongation, including components of the DSIF and NELF complexes. The activities of conventional insulators are also impaired in embryos containing reduced levels of DSIF and NELF. Thus, promoter-proximal stalling factors might help promote insulator-promoter interactions. We propose that stalled promoters help organize gene complexes within chromosomal loop domains.