Site of action of sleep-inducing muramyl peptide isolated from human urine: microinjection studies in rabbit brains.
Site of action of sleep-inducing muramyl peptide isolated from human urine: microinjection studies in rabbit brains.
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从人尿中分离出的诱导睡眠胞壁酰肽的作用位点：兔脑中的显微注射研究。
DOI：
10.1152/jn.1983.49.2.528
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发表时间：
1983
影响因子：
2.5
通讯作者：
J. Pappenheimer
中科院分区：
文献类型：
--
作者：
J. García;J. Pappenheimer
1.将从人尿液中提纯的促睡眠多肽(SPU)通过长期埋置的导引管注入未麻醉兔的基底前脑和脑干特定区域。在每次注射后记录脑电(EEG)和身体活动。2.在52个注射部位中，有8个注射SPU引起持续5小时或以上的慢波睡眠(SWS)显著增加。每个活动站点分别用SPU和车辆控制进行了两次测试。3.7个活动部位位于视交叉水平的基底前脑至中间脑交界处。下部脑干未见活性部位。4.微量注射SPU诱发的过度睡眠综合征的行为学和脑电特征正常，与脑室注射睡眠因子后的过度睡眠相似。然而，在活性部位注射后的反应比侧脑室注射后的反应更快。
1. Sleep-promoting muramyl peptide purified from human urine (SPU) was injected through chronically implanted guide tubes into defined regions of the basal forebrain and brain stem of unanesthetized rabbits. Electroencephalogram (EEG) and body movements were recorded subsequent to each injection. 2. Of 52 injection sites, there were 8 in which microinjection of SPU induced significant increases of slow-wave sleep (SWS) for 5 or more hours. Each active site was tested twice with SPU and once with vehicle control. 3. Seven of the active sites were located in a region extending from the basal forebrain at the level of the optic chiasm to the mesodiencephalic junction. No active sites were found in the lower brain stem. 4. The behavioral and EEG characteristics of the excess SWS induced by microinjection of SPU appeared normal and similar to the excess sleep that follows intraventricular infusions of sleep factor. However, the responses occurred sooner after injections into an active site than after infusion into a lateral ventricle.