FATTY-ACID SYNTHESIS IN MITOCHONDRIA OF EUGLENA-GRACILIS
FATTY-ACID SYNTHESIS IN MITOCHONDRIA OF EUGLENA-GRACILIS
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DOI：
10.1111/j.1432-1033.1984.tb08258.x
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
KITAOKA, S
中科院分区：
文献类型：
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作者：
INUI, H;MIYATAKE, K;KITAOKA, S
与其它亚细胞组分不同，大肠杆菌线粒体中存在一个不依赖丙二酰辅酶A的脂肪酸合成系统。股薄肌该系统具有直接从乙酰辅酶A作为引物和C2供体使用NADH作为电子供体合成脂肪酸的能力。脂肪酸通过逆转β-氧化，除了烯酰辅酶A还原酶代替酰基辅酶A脱氢酶在降解系统中起作用。烯酰辅酶A还原酶对各种烯酰辅酶A底物（C4-C12）与NADH或NADPH均具有相当高的活性。在眼虫线粒体中发现了三种链长特异性不同的烯酰辅酶A还原酶，其中一种对巴豆酰辅酶A具有高度特异性。线粒体脂肪酸合成系统有助于蜡酯发酵，这是生物体中发现的厌氧能量产生系统。
A malonyl-CoA-independent fatty acid synthetic system, different from the systems in other subcellular fractions, occurred in mitochondria of E. gracilis. The system had the ability to synthesize fatty acids directly from acetyl-CoA as both primer and C2 donor using NADH as an electron donor. Fatty acids were synthesized by reversal of .beta.-oxidation with the exception that enoyl-CoA reductase functioned instead of acyl-CoA dehydrogenase in the degradation system. A fairly high activity of enoyl-CoA reductase was found on various enoyl-CoA substrates (C4-C12) with NADH or NADPH. Three species of enoyl-CoA reductase, distinct from each other by their chain-length specificity, were found in Euglena mitochondria, and one of them was highly specific for crotonyl-CoA. The mitochondrial fatty-acid synthetic system contributes to wax ester fermentation, the anaerobic energy-generating system found in the organism.