Association of calcium phosphate and fibroblast growth factor-2: a dynamic light scattering study
Association of calcium phosphate and fibroblast growth factor-2: a dynamic light scattering study
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DOI：
10.1002/mabi.200350037
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发表时间：
2004-01-21
影响因子：
4.6
通讯作者：
Kobayashj, N
中科院分区：
文献类型：
--
作者：
Onuma, K;Kanzaki, N;Kobayashj, N
用动态光散射技术研究了成纤维细胞生长因子-2（FGF-2）在中性水溶液中的粒径分布。我们发现FGF-2在0.1-1.0 mg·mL（-1）浓度范围内以二聚体或三聚体形式分布。与流体动力学半径约90 nm的聚集体与此共存，其比例随浓度的降低而增加。在较低浓度下（小于0.10 mg.mL（-1）），平均半径为80-100 nm的FGF-2聚集体占主导地位，并稳定超过一天。将这些FGF-2溶液与磷酸钙溶液混合以产生亚微米尺寸的FGF-2和羟基磷灰石的复合物，其可用作具有骨形成药理学功能的生物植入物。通过利用从无定形磷酸钙到羟基磷灰石的转化，FGF-2被有效地掺入到羟基磷灰石的多晶中。
The size distributions of fibroblast growth factor-2 (FGF-2) in aqueous solutions with neutral pH were investigated with dynamic light scattering technique. We found that the FGF-2 was distributed in dimer or trimer form at concentration of 0.1-1.0 mg.mL(-1). An aggregate with a hydrodynamic radius approximately 90 nm coexisted with this and its proportion increased with a decrease in concentration. At lower concentrations (less than 0.10 mg.mL(-1)) FGF-2 aggregates with an average radius of 80-100 nm were dominant and were stable for more than a day. These FGF-2 solutions were mixed with calcium phosphate solutions to produce a sub-micron sized compound of FGF-2 and hydroxyapatite, which could be used as a biological implant that possessed a pharmacological function for bone formation. By utilizing a transformation from amorphous calcium phosphate to hydroxyapatite, FGF-2 was effectively incorporated into polycrystals of hydroxyapatite.