Coexpression of pinopodes and leukemia inhibitory factor, as well as its receptor, in human endometrium
Coexpression of pinopodes and leukemia inhibitory factor, as well as its receptor, in human endometrium
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DOI：
10.1016/s0015-0282(02)04830-6
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发表时间：
2003-03-01
影响因子：
6.7
通讯作者：
Landgren, BM
中科院分区：
文献类型：
--
作者：
Aghajanova, L;Stavreus-Evers, A;Landgren, BM
目的：确定白血病抑制因子（LIF）和LIF受体（LIFR）蛋白相对于人子宫内膜标本胞饮突形成的细胞类型特异性表达。设计：前瞻性临床研究。设定：以医院为基础的生殖健康单位和大学附属的生殖研究实验室。患者：26名月经周期正常的健康育龄妇女。主要结果测量：获得常规血液和尿液样本，并进行阴道超声检查和子宫内膜活检。结果：LH第6 ~ 9天子宫内膜标本中均存在不同发育阶段的胞饮突，且胞饮突的形成与LIF、LIFR的表达密切相关。表面和腺上皮细胞表达最高水平的LIF和LIFR蛋白，在活检样品中显示完全发育的胞饮突。LIF的免疫染色在腺上皮中更强烈，而LIFR的免疫染色在表面上皮中最强烈。在胞饮突出现之前和之后，LIF和LIFR免疫染色不太强烈或微弱。结论：正常妇女子宫内膜组织中胞饮突、LIF和LIFR蛋白在空间和时间上同时表达，提示细胞分子和结构的改变在胚泡着床的启动中起重要作用。(C)2003年，美国生殖医学会。
Objective: To determine cell-type-specific expression of leukemia inhibitory factor (LIF) and LIF receptor (LIFR) proteins relative to formation of pinopodes in human endometrial samples.Design: Prospective clinical study. Setting: Hospital-based unit for reproductive health and university-affiliated reproductive research laboratories.Patient(s): Twenty-six healthy fertile women with normal menstrual cycles.Main Outcome Measure(s): Routine blood and urine samples were obtained, and vaginal ultrasonography and endometrial biopsy were performed. Pinopode formation and expression of LIF and LIFR were examined in endometrial samples.Result(s): Samples obtained during LH days 6 through 9 had pinopodes at different developmental stages. Both surface and glandular epithelial cells expressed maximal levels of LIF and LIFR protein, in biopsy samples showed fully developed pinopodes. Immunostaining of LIF was more intense in the glandular epithelium, whereas immunostaining of LIFR was most intense in the surface epithelium. Before and after the appearance of pinopodes, LIF and LIFR immunostaining was less intense or faint. Stromal endometrial cells showed faint LIF accumulation.Conclusion(s): The simultaneous positive spatial and temporal expression of pinopodes and LIF and LIFR proteins in endometrial samples from healthy women suggests that both molecular and structural cell changes are important in the initiation of human blastocyst implantation. (C) 2003 by American Society for Reproductive Medicine.