Cloning, expression, and localization analysis of the sucrose transporter gene JcSUT4 in the biofuel plant Jatropha curcas
Cloning, expression, and localization analysis of the sucrose transporter gene JcSUT4 in the biofuel plant Jatropha curcas
复制标题
生物燃料植物麻风树蔗糖转运蛋白基因 JcSUT4 的克隆、表达和定位分析
DOI：
10.1080/13416979.2018.1506249
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发表时间：
2018-01-01
影响因子：
1.5
通讯作者：
Peng, Changcao
中科院分区：
文献类型：
--
作者：
Yang, Ziwei;Liu, Yingbo;Peng, Changcao
摘要蔗糖转运蛋白在植物果实生长发育过程中起着重要的调节作用。麻疯树是一种重要的热带树种，用作生物燃料材料，开花后果实很快成熟。在此，我们克隆并分析了魔芋的Sut基因JcSUT4。JcSUT4编码500个氨基酸残基，经系统发育分析为III型SUT同源物。原生质体瞬时表达分析表明，JcSUT4主要定位于质膜。对酵母蔗糖吸收缺陷突变株的补充表明，JcSUT4具有转运蔗糖的能力。JcSUT4在成熟的源叶和盛花后30d(DAFB)的果实中高表达，这与果实膨大和成熟的阶段一致。本研究表明，JcSUT4基因可能参与了魔芋果实的生长发育，特别是参与了果实中蔗糖的贮藏。综上所述，我们的结果为麻疯树中SUT的分子机制提供了新的见解。
ABSTRACT Sucrose transporters (SUTs) play an important role in regulating fruit growth and development in planta. The fruit of Jatropha curcas, an important tropical tree used as biofuel material, ripens rapidly after flowering. Here, a SUT gene JcSUT4 from J. curcas was cloned and analyzed. JcSUT4, encoding a protein of 500 amino acid residues, was a Type III SUT homolog by a phylogenetic analysis. A transient expression assay in protoplasts showed that JcSUT4 was mainly localized to the plasma membrane. Complementation of yeast sucrose uptake-deficient mutant strains revealed that JcSUT4 was capable of transporting sucrose. JcSUT4 was highly expressed in mature source leaves, as well as in fruits at 30 days after full bloom (DAFB), which was in accordance with the stages of fruit swelling and ripening. This work shows that JcSUT4 may participate in the fruit growth and development of J. curcas, particularly be involved in sucrose storage in fruit. Taken together, our results provide new insights into the molecular mechanisms underpinning of SUTs in Jatropha.