THE DIELECTRIC METHOD OF ESTIMATING PROTEIN HYDRATION
THE DIELECTRIC METHOD OF ESTIMATING PROTEIN HYDRATION
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DOI：
10.1088/0031-9155/2/1/303
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发表时间：
1957-01-01
影响因子：
3.5
通讯作者：
GRANT, EH
中科院分区：
文献类型：
--
作者：
GRANT, EH
测量蛋白质水合作用的介电方法依赖于存在一个频率范围的假设，在该频率范围内，与蛋白质分子结合的水分子不能在施加的电场中转动，而自由水分子仍然可以旋转。因此，纯水的介电常数和蛋白质溶液在该范围内的任何频率下的介电常数之间的差异给出了与单位重量的蛋白质紧密结合的水量的量度。用人血清白蛋白进行研究，得出的结论是，在25-37[degree]C的温度范围内，水合因子约为0.4 g水/g血清白蛋白，上限为0.85。减少误差的方法和提高精度的前景，在未来的工作被认为是。
The dielectric method measuring protein hydration depends on the assumption of the existence of a frequency range where the water molecules bound to the protein molecule are incapable of turning in an applied electric field while the free water molecules can still rotate. Thus the difference between the dielectric constant of pure water and the dielectric constant of the protein solution at any frequency in this range gives a measure of the quantity of water tightly bound to unit weight of protein. The investigations were performed with human serum albumen and it is concluded that the hydration factor in the temperature range 25-37[degree]C is about 0.4 g of water per g of serum albumen with an upper limit of 0.85. Methods of reducing errors and prospects of increasing the accuracy in future work are considered.