Water-deficiency conditions differently modulate the methylome of roots and leaves in barley (Hordeum vulgare L.).
Water-deficiency conditions differently modulate the methylome of roots and leaves in barley (Hordeum vulgare L.).
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DOI：
10.1093/jxb/erv552
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发表时间：
2016-02
影响因子：
6.9
通讯作者：
Kwasniewski M
中科院分区：
文献类型：
--
作者：
Chwialkowska K;Nowakowska U;Mroziewicz A;Szarejko I;Kwasniewski M
水分胁迫和复水后大麦叶和根甲基化组调控的器官特异性反应的表征。基因表达调控是植物适应逆境的重要策略之一，而基因表达调控可能是DNA甲基化调控的结果。本研究试图表征和比较大麦叶片和根系甲基化组在缺水处理和随后的复水阶段。我们的研究结果，使用甲基化敏感的扩增多态性测序分析，表明大麦基因组中的整体DNA甲基化水平高，一般稳定缺水条件下。然而，许多差异甲基化位点（DMS）诱导的压力在叶和根。相同比例的新的压力诱导的甲基化和去甲基化事件中观察到的基因在叶，但新的甲基化占主导地位的根。重复的元素优先进行脱甲基化的叶和新的甲基化的根。重要的是，复水和植物恢复导致大多数胁迫诱导的甲基化事件的可逆性，但这一过程在叶中比在根中更有效。在叶和根的基因区域中鉴定的DMS的子集内富集了不同的生物过程。我们认为，器官特异性的甲基化变化响应水分胁迫可能是一个重要的调节机制，导致大麦的多层次的胁迫耐受机制。
Characterization of barley methylome modulation in leaves and roots under water-deficiency stress and following rewatering with respect to organ-specific responses. One of the strategies of plant adaptation to stress is the modulation of gene expression, which may result from the regulation of DNA methylation. This study attempted to characterize and compare the barley methylome of leaves and roots under water-deficiency treatment and in the subsequent rewatering phase. Our results, obtained using methylation-sensitive amplification polymorphism sequencing analysis, indicated that the overall DNA methylation level in the barley genome was high and in general stable under water-deficiency conditions. Nevertheless, numerous differentially methylated sites (DMSs) were induced by stress in the leaves and roots. Equal proportions of novel stress-induced methylation and demethylation events were observed within the genes in the leaves, but new methylations dominated in the roots. Repetitive elements preferentially underwent demethylation in the leaves and novel methylations in the roots. Importantly, rewatering and plant recovery resulted in the reversibility of the majority of stress-induced methylation events, but this process was more efficient in the leaves than in the roots. Different biological processes were enriched within the subsets of the DMSs that were identified in the genic regions of leaves and roots. We assume that the organ specificity of the methylome changes in response to water deficiency might be an important regulatory mechanism that leads to multi-level mechanisms of stress tolerance in barley.