The antiinflammatory effects of propofol in endotoxemic rats during moderate and mild hypothermia
The antiinflammatory effects of propofol in endotoxemic rats during moderate and mild hypothermia
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DOI：
10.1007/s00540-007-0533-y
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发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
Taniguch, Takumi
中科院分区：
文献类型：
--
作者：
Kanakura, Hiroko;Taniguch, Takumi
目的.我们以前发现，异丙酚减弱大鼠内毒素血症期间的死亡率和炎症反应;然而，异丙酚是否在低温期间保持其抗炎作用尚未确定。我们研究了异丙酚对中度或轻度低氧血症大鼠内毒素血症的影响。用戊巴比妥钠腹腔内麻醉雄性Wistar大鼠（n = 88），并将其分配到两种方案之一：一种代表中度低温（30度-32 ℃），另一种代表轻度低温（33度-35 ℃）。每种方案均分为4组：A组，大肠杆菌内毒素（Escherichiacoli endotoxin，15 mg.）kg（-1），静脉注射）B组（B）：异丙酚10 mg（.）kg（-1.）h（-1），i.v.）C组注射内毒素15 mg（.）kg（-1），静脉注射）D组：异丙酚10 mg（.）kg（-1.）h（-1），i.v.）内毒素注射后体温降低。然后将大鼠加热或冷却，以维持直肠温度如上6 It。在注射内毒素后6 h评估死亡率。此外，我们还评估了血流动力学、酸碱状态和血浆细胞因子浓度。内毒素血症大鼠出现低血压和代谢性酸中毒以及血浆细胞因子浓度升高。注射内毒素后6 h，中度低温下A-D组的死亡率分别为70%、40%、10%和0%。异丙酚对内毒素血症大鼠的低体温（无论是中度还是轻度）也能降低高死亡率、代谢性酸中毒和细胞因子的升高，但这些作用并不上级单纯低体温。在低温期间，丙泊酚给药没有附加的有益的镇静作用。
Purpose. We previously found that propofol attenuated the mortality rate and inflammatory responses during endotoxemia in rats; however, whether propofol retains its antiinflammatory effects during hypothermia has not been determined. We investigated the effects of propofol on endotoxemic rats subjected to moderate or mild hypothermia.Methods. Male Wistar rats (n = 88) were anesthetized intraperitoneally with pentobarbital sodium and assigned to one of two protocols: one representing moderate hypothermia (30 degrees-32 degrees C) and the other representing mild hypotherima (33 degrees-35 degrees C). Each protocol included four equal-sized groups: group A, Escherichia coli endotoxin (15mg(.)kg(-1), i.v.) and normothermia; group B, propofol (10mg(.)kg(-1.)h(-1), i.v.) and normothermia after endotoxin injection; group C, endotoxin (15mg(.)kg(-1), i.v.) and hypothermia; and group D, propofol (10mg(.)kg(-1.)h(-1), i.v.) and hypothermia after endotoxin injection. Rats then were warmed or cooled to maintain rectal temperatures as above for 6 It. The mortality rate was assessed up to 6h after endotoxin injection. In addition, we assessed hemodynamics, acid-base status, and plasma cytokine concentrations.Results. Endotoxemic rats developed hypotension and metabolic acidosis as well as increased plasma cytokine concentrations. Mortality rates 6h after endotoxin injection were 70%, 40%, 10%, and 0% for groups A-D, respectively, at moderate hypothermia. Propofol administration to endotoxemic rats with hypothermia, whether moderate or mild, also attenuated the high mortality rate, metabolic acidosis, and elevation of cytokines, but these effects were not superior to those of hypothermia alone.Conclusion. During hypothermia, propofol administration does not have additive beneficial antiinflammatory effects.