Determination of histamine in canned tuna by molecularly imprinted polymers-surface enhanced Raman spectroscopy
Determination of histamine in canned tuna by molecularly imprinted polymers-surface enhanced Raman spectroscopy
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DOI：
10.1016/j.aca.2015.10.025
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发表时间：
2015-12-11
影响因子：
6.2
通讯作者：
Lu, Xiaonan
中科院分区：
文献类型：
--
作者：
Gao, Fang;Grant, Edward;Lu, Xiaonan
我们介绍了一种快速、经济、可靠的方法来测定金枪鱼罐头中的组胺水平。分子印迹聚合物（MIPs）是利用组胺与功能单体（甲基丙烯酸）的相互作用，在聚合后的聚合物颗粒上印记特定的结合位点而合成的一种针对组胺的人工抗体。聚氯乙烯（PVC）被用来固定MIPs，得到一种MIPs-PVC膜，作为识别元件，可以特异性地从金枪鱼提取物中分离组胺。金胶体溶液既可以作为从MIPs-PVC膜中提取组胺的洗脱溶剂，又可以作为表面增强拉曼光谱（SERS）检测组胺信号的底物。主成分分析结合偏最小二乘回归（PLSR）模型（R-2 = 0.947, RMSECV = 3.526）验证了MIPs-PVC-SERS方法用于组胺检测和光谱分析的可靠性。建立线性回归模型，将不同组胺SERS波段的强度与相应的峰值水平联系起来。其中一个这样的模型（使用1576厘米（-1）的波段）在预测金枪鱼组胺含量方面比PLSR模型稍好。我们的结论是，我们的新的MIPs-PVC-SERS方法可以快速可靠地测定金枪鱼罐头肉中3至90 ppm的组胺含量。(C) 2015 Elsevier B.V.版权所有
We introduce a rapid, cost effective and reliable approach to determine histamine level in canned tuna. Molecularly imprinted polymers (MIPs) were synthesized as artificial antibodies towards histamine by utilizing the interaction between histamine and a functional monomer (methacrylic acid) to impress specific binding sites on polymer particles after polymerization. Polyvinyl chloride (PVC) was used to immobilize the MIPs, yielding a MIPs-PVC film that functioned as a recognition element to specifically separate histamine from tuna extract. A gold colloid solution served both as an eluting solvent to extract histamine from MIPs-PVC film and furnish a substrate for surface enhanced Raman spectroscopy (SERS) detection of histamine signals. Principal component analysis together with a partial least square regression (PLSR) model (R-2 = 0.947, RMSECV = 3.526) verified the reliability of MIPs-PVC-SERS approach for the detection and spectral analysis of histamine. Linear regression models were also constructed to relate the intensity of different histamine SERS bands with the corresponding spiking levels. One such model (using a band at 1576 cm(-1)) performed slightly better in predicting histamine content in tuna than the PLSR model. We conclude that our new MIPs-PVC-SERS approach can rapidly and reliably determine histamine at levels from 3 to 90 ppm in canned tuna meat. (C) 2015 Elsevier B.V. All rights reserved.