Expression of a Musashi-Like Gene in Sexual and Asexual Development of the Colonial Chordate Botryllus schlosseri and Phylogenetic Analysis of the Protein Group
Expression of a Musashi-Like Gene in Sexual and Asexual Development of the Colonial Chordate Botryllus schlosseri and Phylogenetic Analysis of the Protein Group
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DOI：
10.1002/jez.b.21431
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发表时间：
2011-12-15
影响因子：
2.2
通讯作者：
Manni, Lucia
中科院分区：
文献类型：
--
作者：
Gasparini, Fabio;Shimeld, Sebastian M.;Manni, Lucia
被囊动物是唯一的脊索动物，具有有性和无性繁殖的物种。其中，群体海鞘Botryllus schlosseri是研究这两种发育途径异同的参考模型。我们在这里说明的表征和表达模式，在两个途径的转录本的基因orthoblasting到Dazap 1。Dazap 1基因编码RNA结合蛋白，属于Musashi-like（Msi-like）组。我们的系统发育分析表明，这些都与其他RNA结合蛋白（Tardbp和几种异质性核核糖核蛋白类型），共享相同的保守的串联RNA识别基序（RRM）的模块化结构域结构。我们还将整个群体归类为来自RRM的单一古代复制品。我们的研究结果还表明，Dazap 1在B胚胎发生和囊胚发生过程中以离散的时空模式表达。施洛塞里。它从不在完全分化的组织中表达，但它位于所有芽组织和胚胎和幼虫的不同时空限定的区域中。这些表达模式可能表明在这两个过程中的不同作用，但一个有趣的关系出现，如果考虑到细胞分裂动力学方面，这表明它与所有组织中的增殖阶段，并提出了与已知的Dazap 1同源基因在其他后生动物的相似性。J. Exp. Zool.（Mol. Dev. Evol.）316：562-573，2011。(C)2011 Wiley Periodicals，Inc.
Tunicates are the unique chordates to possess species reproducing sexually and asexually. Among them, the colonial ascidian Botryllus schlosseri is a reference model for the study of similarities and differences in these two developmental pathways. We here illustrate the characterization and expression pattern during both pathways of a transcript for a gene orthologous to Dazap1. Dazap1 genes encode for RNA-binding proteins and fall into the Musashi-like (Msi-like) group. Our phylogenetic analysis shows that these are related to other RNA-binding proteins (Tardbp and several heterogeneous nuclear ribonucleoproteins types) that share the same modular domain structure of conserved tandem RNA Recognition Motifs (RRMs). We also classify the whole group as derived from a single ancient duplication of the RRM. Our results also show that Dazap1 is expressed with discrete spatiotemporal pattern during embryogenesis and blastogenesis of B. schlosseri. It is never expressed in wholly differentiated tissues, but it is located in all bud tissues and in different spatiotemporally defined territories of embryos and larva. These expression patterns could indicate different roles in the two processes, but an intriguing relationship appears if aspects of cell division dynamics are taken into account, suggesting that it is related to the proliferative phases in all tissues, and raising a similarity with known Dazap1 orthologs in other metazoans. J. Exp. Zool. (Mol. Dev. Evol.) 316: 562-573, 2011. (C) 2011 Wiley Periodicals, Inc.