Effects of perfluorooctane on the retina as a short-term and small amounts remnant in rabbits.
Effects of perfluorooctane on the retina as a short-term and small amounts remnant in rabbits.
复制标题
全氟辛烷作为短期和少量残留物对兔子视网膜的影响。
DOI：
10.18240/ijo.2019.03.05
复制
发表时间：
2019
影响因子：
1.4
通讯作者：
Jin
中科院分区：
文献类型：
--
作者：
Qingchen Li;Jian Yu;Chunhui Jiang;Haohao Zhu;Kun Liu;Jin
目的
目的：观察全氟辛烷（PFO）短期、少量眼内填充对兔视网膜的影响。
方法
选用新西兰大白兔48只，随机分为4组。PFO组接受0.1 mL PFO用于眼科手术或在手术结束时接受0.1 mL F-辛烷;玻璃体切除术（PPV）组的眼睛填充平衡盐溶液，未接受手术干预的眼睛作为对照。分别于术后1、4、12周取眼进行观察。
结果
在显微镜下，内核层（INL）和外核层（ONL）的核计数在所有时间点在四组之间没有差异;然而，仅在12 wk的两个PFO组中，在下视网膜中发现ONL轻微排列紊乱和偶尔的INL空泡化。其中4组在所有时间点的Caspase-3和TNF-α染色结果相似。另外，PFOa和PPV对照组在4周时以及所有三个PPV组在12周时的IL-8均升高;此外，所有三个PPV组在4周和12周时的凋亡指数（%）也相似地升高。
结论
两种PFO在兔眼中耐受性良好，长达12周，这表明它们可以安全地用作术中工具或用于术后短期和少量填塞。
AIM
To investigate changes in the rabbit retina after short-term and small amounts tamponade of perfluorooctane (PFO).
METHODS
New Zealand rabbits were used, and 48 eyes were randomly and evenly assigned into four different groups. The PFO groups received a residue of 0.1 mL of PFO for ophthalmic surgery or 0.1 mL of F-Octane at the end of surgery; eyes from the pars plana vitrectomy (PPV) group were filled with balanced salt solution and those having not received surgical intervention served as controls. Eyes were collected at 1, 4 and 12wk and studied.
RESULTS
Under a microscope, nuclear counts of the inner nuclear layer (INL) and outer nuclear layer (ONL) did not differ among the four groups at all time points; however, slight disarrangement of the ONL and occasional vacuolization of the INL were found in the inferior retina only at 12wk in two PFO groups. Four of the groups had similar results of Caspase-3 and TNF-α staining at all time points. Alternatively, IL-8 was increased in PFOa and PPV control groups at 4wk and in all three PPV groups at 12wk; also, the apoptotic index (%) was similarly increased in all three PPV groups at 4 and 12wk.
CONCLUSION
Both PFOs are well tolerated in rabbit eyes for up to 12wk, which suggests that they can be used safely as intraoperative tools or for short-term and small amounts tamponade after surgery.