Glucose metabolism and neurogenesis in the gerbil hippocampus after transient forebrain ischemia.
Glucose metabolism and neurogenesis in the gerbil hippocampus after transient forebrain ischemia.
复制标题
DOI：
10.4103/1673-5374.189189
复制
发表时间：
2016-08
影响因子：
6.1
通讯作者：
Hwang IK
中科院分区：
文献类型：
--
作者：
Yoo DY;Lee KY;Park JH;Jung HY;Kim JW;Yoon YS;Won MH;Choi JH;Hwang IK
最近的证据表明，葡萄糖转运蛋白3（GLUT 3）在大脑的能量代谢中起着重要作用。大多数先前的研究已经使用局灶性或缺氧性缺血模型进行，并且集中于基于蛋白质和mRNA水平而不是组织水平的GLUT 3表达的变化。在本研究中，我们观察了成年沙土鼠短暂前脑缺血5分钟后不同时间点海马GLUT 3免疫反应性的变化。假手术组海马CA 1区GLUT 3免疫反应较弱，缺血24 h后CA 1区锥体细胞呈时间依赖性增加，缺血2 ~ 5 d后CA 1区GLUT 3免疫反应明显下降，缺血10 d后CA 1区GLUT 3免疫反应呈高水平。在使用GLUT 3和胶质细胞酸性蛋白（GFAP）的双重免疫荧光研究中，我们观察到星形胶质细胞中的GLUT 3免疫反应性很强。GLUT 3免疫反应性在缺血后增加，并在缺血/再灌注后7天在齿状回达到峰值。在使用GLUT 3和doublecortin（DCX）的双重免疫荧光研究中，我们观察到低水平的GLUT 3免疫反应性分化的成神经细胞的齿状回颗粒下层缺血后。假手术组GLUT 3免疫反应主要位于齿状回颗粒下层。这些结果表明，GLUT 3免疫反应性的增加可能是一种代偿机制，以调节海马CA 1区的葡萄糖水平，并促进成年的齿状回神经发生。
Recent evidence exists that glucose transporter 3 (GLUT3) plays an important role in the energy metabolism in the brain. Most previous studies have been conducted using focal or hypoxic ischemia models and have focused on changes in GLUT3 expression based on protein and mRNA levels rather than tissue levels. In the present study, we observed change in GLUT3 immunoreactivity in the adult gerbil hippocampus at various time points after 5 minutes of transient forebrain ischemia. In the sham-operated group, GLUT3 immunoreactivity in the hippocampal CA1 region was weak, in the pyramidal cells of the CA1 region increased in a time-dependent fashion 24 hours after ischemia, and in the hippocampal CA1 region decreased significantly between 2 and 5 days after ischemia, with high level of GLUT3 immunoreactivity observed in the CA1 region 10 days after ischemia. In a double immunofluorescence study using GLUT3 and glial-fibrillary acidic protein (GFAP), we observed strong GLUT3 immunoreactivity in the astrocytes. GLUT3 immunoreactivity increased after ischemia and peaked 7 days in the dentate gyrus after ischemia/reperfusion. In a double immunofluorescence study using GLUT3 and doublecortin (DCX), we observed low level of GLUT3 immunoreactivity in the differentiated neuroblasts of the subgranular zone of the dentate gyrus after ischemia. GLUT3 immunoreactivity in the sham-operated group was mainly detected in the subgranular zone of the dentate gyrus. These results suggest that the increase in GLUT3 immunoreactivity may be a compensatory mechanism to modulate glucose level in the hippocampal CA1 region and to promote adult neurogenesis in the dentate gyrus.