Antibacterial Activity of Light-Activated Silicone Containing Methylene Blue and Gold Nanoparticles of Different Sizes
Antibacterial Activity of Light-Activated Silicone Containing Methylene Blue and Gold Nanoparticles of Different Sizes
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DOI：
10.1007/s10876-010-0319-5
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发表时间：
2010-09-01
影响因子：
2.8
通讯作者：
Parkin, Ivan P.
中科院分区：
文献类型：
--
作者：
Perni, Stefano;Piccirillo, Clara;Parkin, Ivan P.
使用溶胀-包封-收缩技术，将亚甲蓝和亚甲蓝-金纳米颗粒混合物包封在硅酮聚合物中。当对大肠杆菌和表皮葡萄球菌进行测试并暴露于激光（660 nm）时，所述材料的抗菌性能受到Au纳米颗粒的存在和尺寸的显著影响。使用Weibull灭活模型分析细菌灭活数据。对于E. coli和革兰氏阳性菌S.当存在约2nm直径的Au纳米颗粒时，该参数的值降低，该参数的值指示实现活细胞计数的第一个log（10）减少所需的时间。较大的Au纳米颗粒（直径为5和20 nm）与亚甲蓝的组合也包埋在硅酮中。这些材料的值随着纳米颗粒直径的增加而增加，表明抗菌活性降低。在所有情况下E.大肠杆菌比S.表皮。
Methylene Blue and methylene blue-gold nanoparticle mixtures were encapsulated in a silicone polymer using a swell-encapsulation-shrink technique. The antibacterial properties of the materials, when tested against Escherichia coli and Staphylococcus epidermidis, and exposed to laser light (660 nm), were significantly affected by both the presence and size of Au nanoparticles. Bacterial inactivation data were analysed using the Weibull inactivation model. For both E. coli and S. epidermidis the value of the parameter, indicating the time required to achieve the first log(10) reduction in the viable count, decreased when Au nanopartidies of ca 2 nm diameter were present. Larger Au nanoparticles (diameters of 5 and 20 nm) in combination with methylene blue were also embedded in silicone. The values of these materials increased with nanoparticle diameter, indicating a reduction in antibacterial activity. In all cases E. coli had higher values than S. epidermidis.