Regulation of trophoblast invasion by IL-1β and TGF-β1
Regulation of trophoblast invasion by IL-1β and TGF-β1
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DOI：
10.1034/j.1600-0897.2002.01151.x
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发表时间：
2002-10-01
影响因子：
3.6
通讯作者：
Das, C
中科院分区：
文献类型：
--
作者：
Karmakar, S;Das, C
目的：探讨IL-1β和转化生长因子-β1对滋养层细胞源性蛋白水解酶及其抑制物在mRNA和蛋白水平的影响，以阐明滋养层细胞侵袭的机制。方法：免疫组织化学方法检测滋养层细胞衍生蛋白及其抑制物在人胎盘绒毛中的表达，RT-PCR法和酶谱法研究其在mRNA和蛋白水平对培养的滋养层细胞的调控。结果：滋养层细胞中的滋养层蛋白、基质金属蛋白酶(MMP2、MMP9)、膜型基质金属蛋白酶(MT-MMP1&2)和尿激酶型纤溶酶原激活物(UPA)均受IL-1上调。TIMP-1和PAI-1及PAI-1和PAI-2被转化生长因子-β1上调。结论：滋养层细胞侵袭的时空调控是这两种酶及其同源抑制物平衡相互作用的结果。IL-1β和转化生长因子-β1似乎是调节蛋白水解酶网络从而有效控制滋养细胞可能侵入母体子宫内膜的程度的关键。
PROBLEM: To investigate the effect of IL-1beta and TGF-beta1 on trophoblast derived proteases and their inhibitors at the mRNA and protein level for elucidation of the mechanism of trophoblast invasion.METHOD OF STUDY: Trophoblast derived proteases and their inhibitors were localized in human placental villi by immunohistochemistry, their regulation at mRNA and protein levels were studied using RT-PCR and zymography, respectively, on trophoblast cells in culture.RESULTS: Trophoblast proteases, matrix metalloproteases (MMP-2, MMP-9), membrane type matrix metalloproteases (MT-MMP1&2) and urokinase type plasminogen activator (uPA) were found to be up regulated by IL-1beta while the protease inhibitors, tissue inhibitor of metalloproteases (TIMP-1 & 2) and plasminogen activator inhibitors (PAI-1 & 2) to be up regulated by TGF-beta1.CONCLUSION: Temporo-spatial regulation of trophoblast invasion is the outcome of a balanced interplay between the proteases and their cognate inhibitors. IL-1beta and TGF-beta1 seem to be critical for regulating the protease network thereby effectively controlling the extent to which the trophoblast may invade the maternal endometrium.