Brain Adenosine Production in the Rat during 60 Seconds of Ischemia
Brain Adenosine Production in the Rat during 60 Seconds of Ischemia
复制标题
大鼠缺血 60 秒期间脑腺苷的产生
DOI：
10.1161/01.res.45.4.486
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发表时间：
1979
影响因子：
20.1
通讯作者：
R. Berne
中科院分区：
文献类型：
--
作者：
H. Winn;R. Rubio;R. Berne
在大鼠中，通过切断主动脉，突然改变脑灌注压，以确定缺血脑中腺苷及其代谢产物肌苷和次黄嘌呤的产生。分别于缺血0、5、10、15、30、60秒取脑组织标本。同时测定了ATP、ADP、AMP、磷酸肌酸(PCR)、乳酸和丙酮酸。连续监测血压，并在诱导缺血前测量动脉血氧分压、二氧化碳分压和pH值。腺苷在5秒时由对照组的0.96±0.07升至2.30±0.31(Sti)nmol/g。腺苷显著升高持续60s(5.50±1.24)秒。此外，肌苷在整个缺血60s内呈递增趋势，而次黄嘌呤在缺血60s(31.81±2.01nmol/g)至60s(34.72±2.93)秒之间无明显变化。在最初的5秒内，PCR值下降了1.24fimol/g。开始降压后，AMP和ADP直到30s才有明显变化，而ATP和丙酮酸直到60s才有明显变化，乳酸升高了10s。脑缺血后5秒内脑腺苷的迅速升高支持了腺苷在脑血流调节中的作用。矿石资源45：486-492,1979
In rats, cerebral perfusion pressure was altered abruptly by aortic transection to determine the production by ischemic brain of adenosine and its metabolites, inosine and hypoxanthine. Brain samples were obtained after 0, 5, 10, 15, 30, and 60 seconds of ischemia. Also measured were ATP, ADP AMP, phosphocreatine (PCr), lactate, and pyruvate. Blood pressure was monitored continuously, and arterial Po2, PcO2, and pH were measured just prior to induction of ischemia. Adenosine was elevated to 2.30 ± 0.31 (Sti) nmol/g at 5 seconds from a control value of 0.96 ± 0.07. A significant elevation of adenosine continued to 60 seconds (5.50 ± 1.24). Furthermore, inosine showed a progressive upward trend during the entire 60 seconds of ischemia, whereas no change in hypoxanthine occurred between the moment of transection (31.81 ± 2.01 nmol/g) and 60 seconds of ischemia (34.72 ± 2.93). PCr decreased by 1.24 fimol/g within the first 5 seconds. After the onset of hypotension, significant changes did not occur in AMP and ADP until 30 seconds, and in ATP and pyruvate until 60 seconds after aortic transection; lactate was elevated by 10 seconds. The rapid rise of cerebral adenosine within 5 seconds after the onset of ischemia supports a role for adenosine in the regulation of cerebral blood flow. Ore Res 45: 486-492, 1979