Expression of CD44, CD24 and ESA in pancreatic adenocarcinoma cell lines varies with local microenvironment
Expression of CD44, CD24 and ESA in pancreatic adenocarcinoma cell lines varies with local microenvironment
复制标题
胰腺癌细胞系中CD44、CD24和ESA的表达随局部微环境的不同而变化。
DOI：
10.1016/s1499-3872(11)60073-8
复制
发表时间：
2011-08-01
影响因子：
3.3
通讯作者：
Wang, Chun-You
中科院分区：
文献类型：
--
作者：
Wei, Hong-Ji;Yin, Tao;Wang, Chun-You
背景：新的证据表明胰腺癌是由胰腺癌干细胞分级组织和维持的。此外，消除这些细胞是可能的，并且具有治疗相关性。为探讨胰腺癌干细胞表面标志物CD 44、CD 24和ESA在胰腺癌细胞系中的表达规律及局部微环境对其表达的影响，采用流式细胞术检测5种胰腺癌细胞系（PANC-1、PC-2、MIA-Paca-2、AsPC-1和BxPC-3）中CD 44、CD 24和ESA的表达规律。此外，评估了四种细胞系（PANC-1、PC-2、MIA-Paca-2和BxPC-3）在无血清培养基中形成球体的能力。然后，当肿瘤细胞球形成时进行相同的测定。结果：PANC-1和MIA-Paca-2细胞球中均检测到CD 44和CD 24的表达。在PC-2、MIA-Paca-2和AsPC-1中仅检测到CD 44表达。在BxPC-3中检测到CD 44、CD 24和ESA。肿瘤细胞球在无血清培养基中的PANC-1和MIA-Paca-2中形成。这伴随着PANC-1中CD 24表达的增加和CD 44表达的减少。有趣的是，一旦培养基从无血清培养基变回含血清培养基，CD 44和CD 24的表达就恢复到初始水平。在MIA-Paca-2中未检测到CD 44表达的显著变化。结论：胰腺癌干细胞表面标志物CD 44、CD 24和ESA在不同胰腺癌细胞系中的表达模式不同，并随胰腺癌细胞系局部微环境的改变而改变。
BACKGROUND: Emerging evidence suggests that pancreatic adenocarcinoma is hierarchically organized and sustained by pancreatic cancer stem cells. Furthermore, elimination of these cells is possible and therapeutically relevant. This study aimed to investigate the expression patterns of pancreatic cancer stem cell surface markers CD44, CD24 and ESA in pancreatic adenocarcinoma cell lines and explore the influence of their local microenvironment.METHODS: Flow cytometry was used to analyze the expression patterns of CD44, CD24 and ESA in five pancreatic adenocarcinoma cell lines (PANC-1, PC-2, MIA-Paca-2, AsPC-1 and BxPC-3). In addition, the capacity for sphere-formation in serum-free medium of four cell lines (PANC-1, PC-2, MIA-Paca-2 and BxPC-3) was assessed. Then, the same assays were performed when tumor cell spheres were developed. The role of sonic hedgehog (SHH) in cell spheres from PANC-1 and MIA-Paca-2 were also assessed by RT-PCR.RESULTS: CD44 and CD24 were detected in PANC-1. Only CD44 expression was detected in PC-2, MIA-Paca-2 and AsPC-1. CD44, CD24 and ESA were all detected in BxPC-3. Tumor cell spheres developed in PANC-1 and MIA-Paca-2 in serum-free medium. This was accompanied by an increase in CD24 expression and a decrease in CD44 expression in PANC-1. Interestingly, the expression of CD44 and CD24 returned to initial levels once the medium was changed back from serum-free to serum-containing medium. No significant change in the expression of CD44 was detected in MIA-Paca-2. Furthermore, the relative quantification of SHH mRNA in PANC-1 cell spheres was significantly higher than that in cells cultured in the serum-containing medium.CONCLUSION: The expression patterns of the pancreatic cancer stem cell surface markers CD44, CD24 and ESA were diverse in different pancreatic adenocarcinoma cell lines and changed with their local microenvironment.