Antimicrobial chitosan-PVA hydrogel as a nanoreactor and immobilizing matrix for silver nanoparticles
Antimicrobial chitosan-PVA hydrogel as a nanoreactor and immobilizing matrix for silver nanoparticles
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DOI：
10.1007/s13204-012-0080-1
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发表时间：
2012-09-01
影响因子：
--
通讯作者：
Mukherji, Suparna
中科院分区：
文献类型：
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作者：
Agnihotri, Shekhar;Mukherji, Soumyo;Mukherji, Suparna
水凝胶是能够保留大量水的水不溶性交联亲水网络。本工作旨在开发一种新型的壳聚糖-聚乙烯醇基水凝胶，它既可以作为纳米反应器，也可以作为银纳米颗粒（AgNPs）的固定基质，具有很好的抗菌应用前景。通过使用不同量的交联剂重复冻融处理，然后用硼氢化钠作为还原剂原位还原，制备含有AgNP的水凝胶。表征研究证实，该水凝胶提供了纳米颗粒在聚合物网络内的受控和均匀分布，而无需添加任何其他稳定剂。根据HR-TEM研究，发现平均粒度为13 nm，粒度分布为8至21 nm。溶胀研究证实，凝胶基质内更高量的交联剂和银掺入分别显著增强了水凝胶的聚合物物质的孔隙率和链缠结。的AgNP-水凝胶表现出良好的抗菌活性，并发现在12小时内导致微生物生长（大肠杆菌）的显着减少，而没有AgNP的水凝胶没有观察到这样的活性。
Hydrogels are water-insoluble crosslinked hydrophilic networks capable of retaining a large amount of water. The present work aimed to develop a novel chitosan-PVA-based hydrogel which could behave both as a nanoreactor and an immobilizing matrix for silver nanoparticles (AgNPs) with promising antibacterial applications. The hydrogel containing AgNPs were prepared by repeated freeze-thaw treatment using varying amounts of the crosslinker, followed by in situ reduction with sodium borohydride as a reducing agent. Characterization studies established that the hydrogel provides a controlled and uniform distribution of nanoparticles within the polymeric network without addition of any further stabilizer. The average particle size was found to be 13 nm with size distribution from 8 to 21 nm as per HR-TEM studies. Swelling studies confirmed that higher amount of crosslinker and silver incorporation inside the gel matrices significantly enhanced the porosity and chain entanglement of the polymeric species of the hydrogel, respectively. The AgNP-hydrogel exhibited good antibacterial activity and was found to cause significant reduction in microbial growth (Escherichia coli) in 12 h while such activity was not observed for the hydrogel without AgNPs.