Neohesperidin attenuates obesity by altering the composition of the gut microbiota in high-fat diet-fed mice
Neohesperidin attenuates obesity by altering the composition of the gut microbiota in high-fat diet-fed mice
复制标题
新橙皮苷通过改变高脂肪饮食喂养小鼠肠道微生物群的组成来减轻肥胖
DOI：
10.1096/fj.201903102rr
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发表时间：
2020-07-30
期刊：
影响因子：
4.8
通讯作者：
Wu, Jiang Wei
中科院分区：
文献类型：
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作者：
Lu, Jun Feng;Zhu, Meng Qing;Wu, Jiang Wei
肥胖和相关代谢紊乱与肠道微生物群失调、肠屏障破坏和慢性炎症有关。新橙皮苷（Neo）是柑橘类水果中富含的一种天然多酚，对多种疾病具有预防和治疗作用。在这里，我们报告了Neo给药减轻了高脂饮食（HFD）小鼠的体重增加，低度炎症和胰岛素抵抗。此外，Neo给药基本上恢复了肠道屏障损伤、代谢性内毒素血症和全身性炎症。粪便样本中16S rRNA基因的测序显示，Neo给药逆转了HFD诱导的肠道微生物群生态失调：肠道微生物群多样性增加，肠道微生物群组成改变（特别是拟杆菌和厚壁菌门的相对丰度）。此外，全身性抗生素治疗消除了Neo在体重控制中的有益作用，表明Neo对肥胖减轻的作用在很大程度上取决于肠道微生物群。更重要的是，我们证明了Neo对肥胖调节的影响可以通过粪便微生物群移植从Neo治疗的小鼠转移到HFD喂养的小鼠。总的来说，我们的数据突出了Neo作为益生元制剂用于减轻肥胖的功效，这意味着肠道微生物群介导Neo有益作用的潜在机制。
Obesity and related metabolic disorders are associated with intestinal microbiota dysbiosis, disrupted intestinal barrier, and chronic inflammation. Neohesperidin (Neo), a natural polyphenol abundant in citrus fruits, is known for its preventative and therapeutic effects on numerous diseases. Here, we report that Neo administration attenuates weight gain, low-grade inflammation, and insulin resistance in mice fed high-fat diet (HFD). Also, Neo administration substantially restores gut barrier damage, metabolic endotoxemia, and systemic inflammation. Sequencing of 16S rRNA genes in fecal samples revealed that Neo administration reverses HFD-induced intestinal microbiota dysbiosis: an increase in the diversity of gut microbiota and alteration in the composition of intestinal microbiota (particularly in the relative abundances of Bacteroidetes and Firmicutes). Furthermore, systemic antibiotic treatment abolishes the beneficial effects of Neo in body weight control, suggesting that the effect of Neo on obesity attenuation largely depends on the gut microbiota. More importantly, we demonstrate that the impact of Neo on the regulation of obesity could be transferred from Neo-treated mice to HFD-fed mice via fecal microbiota transplantation. Collectively, our data highlight the efficacy of Neo as a prebiotic agent for attenuating obesity, implying a potential mechanism for gut microbiota mediated the beneficial effect of Neo.