Epidermal growth factor enhances preimplantation developmental competence of maturing mouse oocytes
Epidermal growth factor enhances preimplantation developmental competence of maturing mouse oocytes
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DOI：
10.1093/humrep/14.12.3060
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发表时间：
1999-12-01
影响因子：
6.1
通讯作者：
Eppig, JJ
中科院分区：
文献类型：
--
作者：
De la Fuente, R;O'Brien, MJ;Eppig, JJ
本研究的目的是确定表皮生长因子（EGF）是否促进小鼠卵母细胞在体内或体外培养的核和胞质成熟。体外成熟（IVM）前，从促性腺激素（PR）或未促性腺激素（UPR）的22日龄小鼠体内分离出处于生发泡（GV）期的卵母细胞，从12日龄小鼠腔前卵泡中分离出体外生长（IVG）的卵母细胞，在无促性腺激素的情况下体外生长10天后成熟将IVG/IVM卵母细胞、IVM卵母细胞和IVG/IVM卵母细胞在添加EGF或EGF的培养基中成熟，（10 ng/ml），促卵泡激素（FSH）当从PR和UPR小鼠中分离卵母细胞-卵丘细胞复合物时，用EGF + FSH的IVM，（10 ng/ml）单独或与FSH（100 ng/ml）联合使用，可增加（P < 0.05）核成熟至中期II的发生率。表皮生长因子也能促进PR雌性卵母细胞的胞质成熟，表现为卵裂至2-细胞期和发育至囊胚期的频率增加EGF能显著提高囊胚细胞数，但仅在FSH缺乏时才有此作用而EGF、FSH或EGF + FSH对UPR小鼠卵母细胞完成植入前发育的百分率无明显影响，但能增加每个囊胚的细胞数。与对照组相比，这些配体也增加了IVG卵母细胞达到中期II的比例（53- 57（25%; P < 0.05），EGF单独或与FSH联合使用时，囊胚形成率（P < 0.05）（分别为23%和28%）与对照组（13%）相比，EGF处理的成熟IVG卵母细胞产生的囊胚细胞比其他IVG组更多提示体外促性腺激素可增加卵丘细胞包裹卵母细胞对EGF的敏感性或反应性。从而促进细胞核和细胞质的成熟。然而，卵母细胞-颗粒细胞复合体在体外生长成为EGF无促性腺激素治疗的反应。因此，在减数分裂成熟过程中，EGF处理促进IVG卵母细胞的核和胞质成熟。
The objective of this study was to determine whether epidermal growth factor (EGF) promotes nuclear and cytoplasmic maturation of mouse oocytes grown in vivo or iii vitro. In-vivo-grown oocytes were isolated at the germinal vesicle (GV) stage from gonadotrophin-primed (PR) or -unprimed (UPR) 22-day-old mice before in-vitro maturation (IVM), In-vitro-grown (IVG) oocytes were isolated from preantral follicles of 12-day-old mice and grown in vitro without gonadotrophins for 10 days before maturation (IVG/IVM oocytes), IVM and IVG/IVM oocytes were matured in medium supplemented with either EGF (10 ng/ml), follicle stimulating hormone (FSH) (100 ng/ml), EGF plus FSH, or with neither ligand (control), When oocyte-cumulus cell complexes were isolated from PR and UPR mice, IVM with EGF (10 ng/ml), alone or in combination with FSH (100 ng/ml), increased (P < 0.05) the incidence of nuclear maturation to metaphase II. Cytoplasmic maturation of oocytes from PR females, manifested as increased frequency of cleavage to the 2-cell stage and development to the blastocyst stage, was also enhanced with EGF (P < 0.05), Moreover, EGF increased the number of cells per blastocyst, but only in the absence of FSH (P < 0.01), In contrast, EGF, FSH, or EGF plus FSH did not affect the percentage of oocytes from UPR mice completing preimplantation development, but did increase the number of cells per blastocyst. These ligands also increased the proportion of IVG oocytes reaching metaphase II (53-57%) compared with controls (25%; P < 0.05), EGF alone or in combination with FSH increased (P < 0.05) the frequency of blastocyst formation (23% and 28%, respectively) compared with controls (13%), EGF treatment of maturing IVG oocytes produced blastocysts with more cells than other IVG groups (P < 0.05), It is concluded that gonadotrophins irt vivo increase the sensitivity or responsiveness of cumulus cell-enclosed oocytes to EGF, thereby promoting both nuclear and cytoplasmic maturation. However, oocyte-granulosa cell complexes grown in vitro become responsive to EGF without gonadotrophin treatment. Thus, nuclear and cytoplasmic maturation of IVG oocytes is promoted by EGF treatment during meiotic maturation.