First report of Echinococcus shiquicus in dogs from eastern Qinghai-Tibet plateau region, China
First report of Echinococcus shiquicus in dogs from eastern Qinghai-Tibet plateau region, China
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DOI：
10.1016/j.actatropica.2013.02.019
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发表时间：
2013-07-01
期刊：
影响因子：
2.7
通讯作者：
Craig, Philip S.
中科院分区：
文献类型：
--
作者：
Boufana, Belgees;Qiu, Jiamin;Craig, Philip S.
2005年在中国四川省的狐狸和鼠兔野生动物宿主中发现了石奎棘球绦虫。用coproPCR方法对四川省甘孜藏族自治州石渠县棘球蚴病净化调查中采集的犬粪便样品进行了检测，以探讨是否存在棘球蚴病。shiquicus。此外，从8只尸检藏狐（Vulpesferrilata）体内提取粪原DNA，并与E. shiquicus也包括在内。30%（6/20）的狗粪便样品为E.在PCR扩增ND 1线粒体基因内的片段后，测序结果证实4只犬感染了志贺氏棘球绦虫，其中3只犬同时感染了志贺氏棘球绦虫。多房的这些也通过测序进行了验证。用PCR方法检测了2只藏狐粪便中的E. multilocularis和E.志贺氏菌DNA其中一个双E。multilocularis和E.通过测序证实了藏狐中的志贺氏菌感染。(C)2013爱思唯尔有限公司版权所有。
Echinococcus shiquicus was discovered in foxes and pika wildlife hosts in Sichuan Province, China in 2005. Faecal samples from dogs collected in a previous echinococcosis purgation survey from Shiqu County, Ganzi Tibetan Autonomous Prefecture (Sichuan) were screened by coproPCR to investigate the possible occurrence of E. shiquicus. In addition, coproDNA extracted from 8 necropsied Tibetan foxes (Vulpes ferrilata), the natural host of E. shiquicus, were also included. Thirty (6/20) percent of faecal samples from dogs were positive for E. shiquicus DNA after PCR amplification of a fragment within the ND1 mitochondrial gene. Echinococcus shiquicus was confirmed by sequencing in four dogs and 3 of the 6 dogs were concurrently infected with E. multilocularis. These were also verified by sequencing. Faecal samples from two Tibetan foxes were shown by PCR to harbour both E. multilocularis and E. shiquicus DNA. One of these dual E. multilocularis and E. shiquicus infections in a Tibetan fox was confirmed by sequencing. (C) 2013 Elsevier B.V. All rights reserved.