IDENTIFICATION OF RAPD MARKERS LINKED TO GENETIC-FACTORS CONTROLLING THE MILLING ENERGY REQUIREMENT OF BARLEY
IDENTIFICATION OF RAPD MARKERS LINKED TO GENETIC-FACTORS CONTROLLING THE MILLING ENERGY REQUIREMENT OF BARLEY
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DOI：
10.1007/bf01184917
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发表时间：
1993-11-01
影响因子：
5.4
通讯作者：
POWELL, W
中科院分区：
文献类型：
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作者：
CHALMERS, KJ;BARUA, UM;POWELL, W
大麦的加倍单倍体群体与基于聚合酶链式反应的多态分析方法相结合，被用来寻找与控制籽粒碾磨能需求的基因连锁的分子标记。碾磨能(ME)是一个数量性状，通过结合代表ME分布的极端成员的DH家系的DNA，定位单个数量性状座位(QTL)涉及到大块构建。此外，这些个体在Rrn2基因座上有其他的等位基因，此前已被证明与ME QTL相关联。利用随机扩增多态性DNA(随机扩增多态性DNA)标记和多态扩增产物对DNA片段进行筛选，以确定其是否与影响ME基因表达的基因连锁。已鉴定出几个标记，这些标记与控制ME的QTL和通过最大似然法确定的重组比例相关联。结果表明，DHS结合RAPDS和混合分离分析是定位大麦QTL的有效方法。此外，这种方法也适用于在一系列生物中定位其他QTL，从这些生物中可以提取出DH或重组自交系。
Doubled haploid (DH) populations of barley have been used in combination with PCR-based polymorphic-assay procedures to identify molecular markers linked to genes controlling the milling energy requirement of the grain. Milling energy (ME) is a quantitative trait and locating individual quantitative trait loci (QTLs) involved the construction of bulks by combining DNA from DH families representing the extreme members of the distribution for ME. In addition, the individuals had alternative alleles at the Rrn2 locus that has previously been shown to be linked to an ME QTL. The DNA bulks were screened with Randomly Amplified Polymorphic DNA (RAPD) markers and polymorphic amplification products tested for linkage to genes influencing the expression of ME in a DH population. Several markers were identified which are linked to a QTL controlling ME and the recombination fraction determined by maximum likelihood procedures. The results indicate that DHs in combination with RAPDs and bulked segregant analysis provide an efficient method for locating QTLs in barely. Furthermore, this approach is applicable to mapping other QTLs in a range of organisms from which DH or recombinant inbred lines can be extracted.