Pelagibacterium xiamenense sp. nov., isolated from intertidal sediment.
Pelagibacterium xiamenense sp. nov., isolated from intertidal sediment.
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DOI：
10.1099/ijsem.0.005462
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发表时间：
2022-08
影响因子：
2.8
通讯作者：
Y. He;Lina Lyu;Chunming Dong;Wanpeng Wang;Zhiqiang Yu;Z. Shao
中科院分区：
文献类型：
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作者：
Y. He;Lina Lyu;Chunming Dong;Wanpeng Wang;Zhiqiang Yu;Z. Shao
从厦门海沧海岸潮滩沉积物中分离到一株革兰氏阴性的专性好氧细菌，命名为HS 1C 4 - 1 T。该菌株呈淡黄色，不滑动，杆状，能动，具有单个极鞭毛。HS 1C 4 - 1 T细胞呈氧化酶和过氧化氢酶阳性。该菌株可在15-55 °C（最佳37 °C）、pH 6.0-10.0（最佳，pH 7.0-9.0）、0- 12%（最佳，1%）NaCl（w/v）存在下生长。系统发育分析表明，HS 1C 4 - 1 T为Pelagibacterium属的一个成员，与Pelagibacterium lutescerus（CGMCC 1.10267T）的同源性最高（97.6%）。HS 1C 4 - 1 T与Pelagibacterium属所有种的DNA-DNA数字杂交值和平均核苷酸同源性分别为18.7- 20.2%和77.3- 78.4%。主要脂肪酸（> 10%）是C19：0环ω 8 c（50.5%）和总特征8（C18：1ω 7 c和/或C18：1ω6c; 29.1%）。Q-10是唯一的呼吸醌。极性脂质包括二磷脂酰甘油、磷脂酰甘油、两种未鉴别的糖脂和六种未鉴别的脂质。染色体DNA的G+C含量为62.9%。根据系统发育、表型、化学分类和基因组特征，HS 1C 4 - 1 T代表Pelagibacterium属中的一个新种，命名为厦门Pelagibacterium xiamenense sp. nov.。模式菌株为HS 1C 4 - 1 T（=MCCC 1A 18759 T =KCTC 92097 T）。
A Gram-stain-negative, obligately aerobic bacterium, designated as HS1C4-1T, was isolated from a sediment sample from the tidal zone of the Haicang Coast, Xiamen, Fujian Province, PR China. The strain was yellowish-coloured, non-gliding, rod-shaped and motile, with a single polar flagellum. Cells of HS1C4-1T were oxidase- and catalase-positive. The strain could grow at 15-55 °C (optimum 37 °C), pH 6.0-10.0 (optimum, pH 7.0-9.0), in the presence of 0-12 % (optimum, 1 %) NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that HS1C4-1T represented a member of the genus Pelagibacterium, and shared the highest similarity to Pelagibacterium luteolum CGMCC 1.10267T (97.6 %). Digital DNA-DNA hybridization values and average nucleotide identity between HS1C4-1T and all the species of genus Pelagibacterium were 18.7-20.2 % and 77.3-78.4 %, respectively. The principal fatty acids (>10 %) were C19 : 0 cyclo ω8c (50.5 %) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 29.1%). Q-10 was the sole respiratory quinone. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and six unidentified lipids. The G+C content of the chromosomal DNA was 62.9 %. On the basis of phylogenetic, phenotypic, chemotaxonomic and genomic characteristics, HS1C4-1T represents a novel species within the genus Pelagibacterium, for which the name Pelagibacterium xiamenense sp. nov. is proposed. The type strain is HS1C4-1T (=MCCC 1A18759T=KCTC 92097T).