[Angiogenesis promoting effects of Chinese herbal medicine for activating blood circulation to remove stasis on chick embryo chorio-allantoic membrane].
[Angiogenesis promoting effects of Chinese herbal medicine for activating blood circulation to remove stasis on chick embryo chorio-allantoic membrane].
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发表时间：
2005-10
期刊：
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通讯作者：
D. Gao;Jun Song;Juan Hu;Jiumao Lin;Liang-pu Zheng;Jing Cai;Jian Du;Ke-Ji Chen
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文献类型：
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作者：
D. Gao;Jun Song;Juan Hu;Jiumao Lin;Liang-pu Zheng;Jing Cai;Jian Du;Ke-Ji Chen
目的观察临床常用活血化瘀中药对鸡胚绒毛尿囊膜血管生成的促进作用。方法建立鸡胚CAM模型，分别应用含赤芍、党参、红花、黄芪、丹参、黄芪及其复方当归补血汤、血府逐瘀汤、雄芍胶囊的小鼠血清，孵育72 h后观察血管生成情况。另设生理盐水组、空白血清组、空白对照组和碱性成纤维细胞生长因子（bFGF）组作为对照。结果中药组和bFGF组均具有促进CAM血管生成的作用，其中黄芪组和3个复方组在毛细血管形成和计数方面均优于3个阴性对照组，与bFGF组作用相似。除丹参外，复方的作用均优于单味药，但上级。结论丹参、当归补血汤、血府逐瘀汤、雄芍胶囊对CAM有较好的促血管生成作用。本研究从一定角度阐明了中药治疗缺血性疾病的作用机制，为复方应用提供了科学依据。
OBJECTIVE To observe the angiogenesis promoting effects of clinical common used Chinese herbal medicines (CHM) for activating blood circulation to remove stasis on chick embryo chorio-allantoic membrane (CAM). METHODS Chicken CAM model was established and mice blood serum containing different kinds of medicines, including Radix Peaoniae rubra, Radix Angelicae sinensis, Flos Carthami, Rhizoma Chuanxiong, Radix Salviae miltiorrhizae, Astragalus membranaceus, and their complex prescriptions, Danggui Buxue Decoction, Xuefu Zhuyu Decoction, Xiongshao Capsule, was applied on it respectively to observe the condition of angiogenesis 72 hrs after incubation. Besides, the normal saline group, blank serum group, blank group and basic fibroblast growth factor (bFGF) group were set up for control. RESULTS All the CHM applied and bFGF had the CAM angiogenetic promoting effect, among them, Radix Salviae Miltiorrhizae and the three complex prescriptions showed better effects than the three negative control groups in capillary formation and count, with the efficacy similar to that of bFGF. The effect of complex prescriptions was superior to that of single herb except Radix Salviae miltiorrhizae. CONCLUSION Radix Salviae miltiorrhizae, Danggui Buxue Decoction, Xuefu Zhuyu Decoction and Xiongshao Capsule have good angiogenesis promoting effect on CAM. This study elucidated, from a certain aspect, the mechanism of action of CHM on ischemic diseases, and unfolded the scientific evidence of applying complex prescription.