A two-photon and second-harmonic microscope
A two-photon and second-harmonic microscope
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DOI：
10.1016/s1046-2023(03)00003-3
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发表时间：
2003-05-01
期刊：
影响因子：
4.8
通讯作者：
Yuste, R
中科院分区：
文献类型：
--
作者：
Nikolenko, V;Nemet, B;Yuste, R
双光子显微镜使高散射组织中的活体制剂能够长期成像，同时将光损伤降至最低，从而为生命科学带来了革命性的变化。同时，商用双光子显微镜价格昂贵，这阻碍了这项技术在生物界的广泛应用。作为商业系统的替代方案，我们通过改进奥林巴斯FluoView激光扫描共聚焦显微镜，提供了我们在设计定制双光子仪器方面所做的最新努力。在我们新版本的仪器中，我们使用Pockels单元和软件控制扫描，以任意的时空模式调制激光的强度。我们还可以同时进行光学成像和光学刺激实验，并将它们与二次谐波产生测量相结合。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
Two-photon microscopy has revolutionized life sciences by enabling long-term imaging of living preparations in highly scattering tissue while minimizing photodamage. At the same time, commercial two-photon microscopes are expensive and this has prevented the widespread application of this technique to the biological community. As an alternative to commercial systems, we provide an update of our efforts designing custom-built two-photon instruments by modifying the Olympus FluoView laser scanning confocal microscope. With the newer version of our instrument we modulate the intensity of the laser beam in arbitrary spatiotemporal patterns using a Pockels cell and software control over the scanning. We can also perform simultaneous optical imaging and optical stimulation experiments and combine them with second harmonic generation measurements. (C) 2003 Elsevier Science (USA). All rights reserved.