Detection of HNE Modification of Proteins in Aging Mouse Tissues: A Western Blot-Based Approach.
Detection of HNE Modification of Proteins in Aging Mouse Tissues: A Western Blot-Based Approach.
复制标题
检测衰老小鼠组织中蛋白质的 HNE 修饰：基于蛋白质印迹的方法。
DOI：
10.1007/978-1-0716-0592-9_21
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Forman,HenryJay
中科院分区：
文献类型：
--
作者：
Zhang,Hongqiao;Lyn,Natalie;Haghani,Amin;Forman,HenryJay
4-Hydroxenonenal (HNE) is one of the major α,β-unsaturated aldehyde products of lipid peroxidation. HNE can form conjugates with macromolecules, including protein, and thereby alter their function. HNE and its conjugation with proteins are increased in aging and age-related diseases. To elucidate how HNE is involved in these aging-related pathophysiological changes, it is necessary to assess HNE modification of proteins. Here a simple and convenient Western-blot based method is presented to detect HNE modification of proteins in tissues of aging mice.
