Single pulse heating of a nanoparticle array for biological applications.
Single pulse heating of a nanoparticle array for biological applications.
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DOI：
10.1039/d1na00766a
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发表时间：
2022-05-07
影响因子：
4.7
通讯作者：
Qin, Zhenpeng
中科院分区：
文献类型：
--
作者：
Xie, Chen;Kang, Peiyuan;Cazals, Johan;Castelan, Omar Morales;Randrianalisoa, Jaona;Qin, Zhenpeng
由于能够将外部激发转化为热量，纳米材料在许多生物医学应用中发挥着重要作用。纳米粒子阵列加热的两种模式，纳米尺度限制加热（NCH）和宏观尺度集体加热（MCH），已被发现和广泛的研究。尽管如此，在蛋白质水平上产生的生物反应仍然难以捉摸。在这项研究中，我们开发了一个计算模型，系统地研究单脉冲加热的NP阵列和相应的蛋白质变性/激活。我们发现NCH可能导致靶蛋白变性，然而，纳米颗粒加热不会导致纳米级选择性TRPV 1通道激活。激发持续时间和NP浓度是确定靶蛋白变性窗口的主要因素，并且与加热功率一起，我们定义了靶蛋白变性的定量边界。我们的研究结果提高了我们对现实物理约束下的NCH和MCH的理解，并为定制具有所需NP加热的生物医学平台提供了强有力的指导。本研究主要研究单脉冲加热纳米粒子阵列对生物活性的影响。分析表明，纳米尺度限制的加热可能导致靶向蛋白质变性，而它不会靶向激活热敏TRPV 1通道。
With the ability to convert external excitation into heat, nanomaterials play an essential role in many biomedical applications. Two modes of nanoparticle (NP) array heating, nanoscale-confined heating (NCH) and macroscale-collective heating (MCH), have been found and extensively studied. Despite this, the resulting biological response at the protein level remains elusive. In this study, we developed a computational model to systematically investigate the single-pulsed heating of the NP array and corresponding protein denaturation/activation. We found that NCH may lead to targeted protein denaturation, however, nanoparticle heating does not lead to nanoscale selective TRPV1 channel activation. The excitation duration and NP concentration are primary factors that determine a window for targeted protein denaturation, and together with heating power, we defined quantified boundaries for targeted protein denaturation. Our results boost our understandings of the NCH and MCH under realistic physical constraints and provide robust guidance to customize biomedical platforms with desired NP heating. This study focuses on the effect of single pulse heating of nanoparticle array on biological activity. The analysis demonstrates that nanoscale-confined heating may lead to targeted protein denaturation, whereas it does not give targeted activation of the thermally-sensitive TRPV1 channel.