The effects of intraspinal microstimulation on spinal cord tissue in the rat
The effects of intraspinal microstimulation on spinal cord tissue in the rat
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DOI：
10.1016/j.biomaterials.2010.03.051
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发表时间：
2010-07-01
期刊：
影响因子：
14
通讯作者：
Mushahwar, Vivian K.
中科院分区：
文献类型：
--
作者：
Bamford, Jeremy A.;Todd, Kathryn G.;Mushahwar, Vivian K.
椎管内微刺激（ISMS）涉及将微丝植入损伤水平以下的脊髓中，以刺激参与控制下肢运动的神经网络。本研究的目的是检查慢性 ISMS 可能发生的潜在脊髓损伤。我们采用了连续脊髓切片的力募集和免疫组织化学处理的功能性测量来评估脊髓横断、ISMS 阵列植入和持续 30 天每天 4 小时的电刺激引起的任何损伤。功能测量显示横断和慢性 ISMS 后的力募集没有变化，表明潜在的神经网络没有变化。假椎管内微丝的植入使微丝轨道附近的小胶质细胞/巨噬细胞和 GFAP+ 星形胶质细胞的密度在空间上受到限制，这表明存在持续的免疫反应。最重要的是，这些结果与微线周围的结果没有什么不同，微线周围的电荷水平高达 48 nC/相。同样，神经元密度的测量表明，与假微丝周围的组织（207.8/mm2 +/- 38.8/mm2）相比，ISMS微丝周围的腹侧灰质中的神经元细胞体（243.6/mm2+/-353/mm2）没有减少。我们的结论是，脊髓组织对椎管内微丝的植入和长期应用 ISMS 具有良好的耐受性。 Crown 版权所有 (c) 2010 由 Elsevier Ltd 出版。保留所有权利。
Intraspinal microstimulation (ISMS) involves the implantation of microwires into the spinal cord below the level of an injury to excite neural networks involved in the control of locomotion in the lower limbs. The goal of this study was to examine the potential spinal cord damage that might occur with chronic ISMS. We employed functional measures of force recruitment and immunohistochemical processing of serial spinal cord sections to evaluate any damage induced by spinal transection, implantation of ISMS arrays, and electrical stimulation of 4 h/day for 30 days. Functional measurements showed no change in force recruitment following transection and chronic ISMS, indicating no changes to underlying neural networks. The implantation of sham intraspinal microwires produced a spatially-limited increase in the density of microglia/macrophages and GFAP+ astrocytes adjacent to the microwire tracks, indicating a persistent immune response. Most importantly, these results were not different from those around microwires that were chronically pulsed with charge levels up to 48 nC/phase. Likewise, measurements of neuronal density indicated no decrease in neuronal cell bodies in the ventral grey matter surrounding ISMS microwires (243.6/mm2 +/- 353/mm2) compared to tissue surrounding sham microwires (207.8/mm2 +/- 38.8/mm2). We conclude that the implantation of intraspinal microwires and chronic application of ISMS are well tolerated by spinal cord tissue. Crown Copyright (c) 2010 Published by Elsevier Ltd. All rights reserved.