Cold-Sensitive Corneal Afferents Respond to a Variety of Ocular Stimuli Central to Tear Production: Implications for Dry Eye Disease
Cold-Sensitive Corneal Afferents Respond to a Variety of Ocular Stimuli Central to Tear Production: Implications for Dry Eye Disease
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DOI：
10.1167/iovs.09-4744
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发表时间：
2010-08-01
影响因子：
4.4
通讯作者：
Meng, Ian D.
中科院分区：
文献类型：
--
作者：
Hirata, Harumitsu;Meng, Ian D.
目的.研究角膜传入神经的反应特征，以检测对流泪反射的“传入肢”的激活至关重要的眼部条件。在异氟烷麻醉的雄性大鼠中，在体内细胞外探索三叉神经节，以识别可被对流泪重要的眼部刺激激活的角膜神经元。在验证了它们的感受野位点仅限于角膜后，用各种刺激表征神经反应特性，例如角膜的干燥和润湿，通过施加和去除人工泪液，温度变化（35 ℃-15 ℃和39 ℃-51 ℃）、薄荷醇（10-100 μ M）和高渗溶液（NaCl、蔗糖; 297-3014 mOsm），应用于眼表面。一种特殊类型的角膜传入被确定为眼表干燥的反应。这些神经元被归类为无害的“冷”的温度感受器，它们对稳态和动态温度变化施加到角膜。除了干燥和轻微冷却外（
PURPOSE. To investigate the response characteristics of the corneal afferents that detect ocular conditions critical to the activation of the "afferent limb" of the lacrimation reflex.METHODS. In isoflurane-anesthetized male rats, trigeminal ganglia were explored extracellularly in vivo to identify the corneal neurons that can be activated by ocular stimuli important to lacrimation. After verifying their receptive field loci to be restricted to the cornea, neural response properties were characterized with a variety of stimuli, such as drying and wetting of the cornea, by applying and removing artificial tears, temperature changes (35 degrees C-15 degrees C and 39 degrees C-51 degrees C), menthol (10-100 mu M), and hyperosmolar solutions (NaCl, sucrose; 297-3014 mOsm), applied to the ocular surface.RESULTS. A specific type of corneal afferent was identified that responded to drying of the ocular surface. These neurons were classified as innocuous "cold" thermoreceptors by their responses to steady state and dynamic temperature changes applied to the cornea. In addition to drying and slight cooling (