Effects of low-molecular-weight organic ligands and phosphate on DNA adsorption by soil colloids and minerals
Effects of low-molecular-weight organic ligands and phosphate on DNA adsorption by soil colloids and minerals
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DOI：
10.1016/j.colsurfb.2006.07.013
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发表时间：
2007-01-15
影响因子：
5.8
通讯作者：
Liang, Wei
中科院分区：
文献类型：
--
作者：
Cai, Peng;Huang, Qiaoyun;Liang, Wei
在柠檬酸盐、酒石酸盐和磷酸盐存在下，研究了DNA在蒙脱土、高岭石、针铁矿和土粘土上的吸附。明显减少观察DNA吸附在蒙脱石和高岭石与增加负离子浓度从0到5毫米。然而,DNA吸附量由蒙脱石和高岭石是增强配体浓度高于5毫米。在土壤胶体和针铁矿的系统,随着离子浓度的增加,稳定降低被发现和配体的能力令人沮丧的DNA吸附后序列:磷酸盐>柠檬酸>酒石酸。与h2o2处理过的粘土（无机粘土）相比，随着配体浓度的增加，针铁矿和有机-矿物复合物（有机粘土）对DNA的吸附量急剧下降。结果表明，阴离子对DNA吸附的影响随阴离子的种类、浓度以及土壤组分的表面性质而异。在加入配体之前将DNA引入体系，土壤胶体和针铁矿对DNA的吸附量最大。在加入DNA之前，有机配体和无机配体对蒙脱土和高岭石的吸附均有促进作用。本研究的结果对于理解DNA在土壤环境中的持久性和命运具有重要意义，特别是在各种有机和无机配体活跃的根际土壤中。(C) 2006 Elsevier B.V.版权所有
Adsorption of DNA on montmorillonite, kaolinite, goethite and soil clays from an Alfisol in the presence of citrate, tartrate and phosphate was studied. A marked decrease in DNA adsorption was observed on montmorillonite and kaolinite with increasing anion concentrations from 0 to 5 mM. However, the amount of DNA adsorbed by montmorillonite and kaolinite was enhanced when ligand concentration was higher than 5 mM. In the system of soil colloids and goethite, with the increase of anion concentrations, a steady decrease was found and the ability of ligands in depressing DNA adsorption followed the sequence: phosphate > citrate > tartrate. Compared to H2O2-treated clays (inorganic clays), a sharp decrease in DNA adsorption was observed on goethite and organo-mineral complexes (organic clays) with increasing ligand concentrations. The results suggest that the influence of anions on DNA adsorption varies with the type and concentration of anion as well as the surface properties of soil components. Introduction of DNA into the system before the addition of ligands had the greatest amount of DNA adsorption on soil colloids and goethite. Organic and inorganic ligands promoted DNA adsorption on montmorillonite and kaolinite when ligands were introduced into the system before the addition of DNA. The results obtained in this study have important implications for the understanding of the persistence and fate of DNA in soil environments especially rhizosphere soil where various organic and inorganic ligands are active. (C) 2006 Elsevier B.V. All rights reserved.