Sorbitol Can Fuel Mouse Sperm Motility and Protein Tyrosine Phosphorylation via Sorbitol Dehydrogenase
Sorbitol Can Fuel Mouse Sperm Motility and Protein Tyrosine Phosphorylation via Sorbitol Dehydrogenase
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DOI：
10.1095/biolreprod.108.068882
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发表时间：
2009-01-01
影响因子：
3.6
通讯作者：
Gerton, George L.
中科院分区：
文献类型：
--
作者：
Cao, Wenlei;Aghajanian, Haig K.;Gerton, George L.
可以代谢产生ATP的能量来源对于正常的精子功能（如运动）至关重要。精液中含有两种主要的单糖，山梨糖醇和果糖。此外，山梨醇脱氢酶（SORD）可以将山梨醇转化为果糖，然后可以通过精子中的糖酵解途径代谢以产生ATP。在这里，我们的特点Sord mRNA和SORD表达在小鼠精子发生和研究山梨醇的能力，以支持附睾精子活力和酪氨酸磷酸化。在精子发生分化过程中，Sord mRNA水平逐渐升高。而SORD蛋白则是在精子细胞凝聚期首次被检测到。间接免疫荧光法显示，SORD沿着附睾尾部精子鞭毛的长度分布。免疫电镜显示SORD与精子的线粒体和质膜结合。与山梨醇孵育的精子保持活力，表明山梨醇被用作能量来源。山梨醇，以及葡萄糖和果糖，是不是必不可少的诱导运动过度活跃。蛋白质酪氨酸磷酸化增加，以类似的方式时，山梨糖醇取代葡萄糖在用于精子获能的培养液。这些结果表明，山梨醇可以作为精子运动和蛋白酪氨酸磷酸化的替代能源。
Energy sources that can be metabolized to yield ATP are essential for normal sperm functions such as motility. Two major monosaccharides, sorbitol and fructose, are present in semen. Furthermore, sorbitol dehydrogenase (SORD) can convert sorbitol to fructose, which can then be metabolized via the glycolytic pathway in sperm to make ATP. Here we characterize Sord mRNA and SORD expression during mouse spermatogenesis and examine the ability of sorbitol to support epididymal sperm motility and tyrosine phosphorylation. Sord mRNA levels increased during the course of spermatogenic differentiation. SORD protein, however, was first detected at the condensing spermatid stage. By indirect immunofluorescence, SORD was present along the length of the flagella of caudal epididymal sperm. Furthermore, immunoelectron microscopy showed that SORD was associated with mitochondria and the plasma membranes of sperm. Sperm incubated with sorbitol maintained motility, indicating that sorbitol was utilized as an energy source. Sorbitol, as well as glucose and fructose, were not essential to induce hyperactive motility. Protein tyrosine phosphorylation increased in a similar manner when sorbitol was substituted for glucose in the incubation medium used for sperm capacitation. These results indicate that sorbitol can serve as an alternative energy source for sperm motility and protein tyrosine phosphorylation.