Cloning, sequencing, chromosomal location, and function of cDNAs encoding an opioid growth factor receptor (OGFr) in humans.
Cloning, sequencing, chromosomal location, and function of cDNAs encoding an opioid growth factor receptor (OGFr) in humans.
复制标题
人类阿片生长因子受体 (OGFr) 编码 cDNA 的克隆、测序、染色体定位和功能。
DOI：
10.1016/s0006-8993(99)02330-6
复制
发表时间：
2000
期刊：
影响因子：
2.9
通讯作者：
McLaughlin,PJ
中科院分区：
文献类型：
--
作者：
Zagon,IS;Verderame,MF;Allen,SS;McLaughlin,PJ
天然阿片生长因子 (OGF)，[Met5]-脑啡肽，是一种强效抑制肽，可在发育、癌症、细胞更新、伤口愈合和血管生成过程中调节细胞增殖和组织组织。 OGF 的作用是由受体机制介导的。我们已经克隆并测序了编码人类 OGF 受体多种剪接形式的 cDNA。最长的 cDNA 中的开放阅读框被发现编码 697 个氨基酸的蛋白质，其中 8 个不完美重复（每个重复 20 个氨基酸）是一个显着特征。总共观察到五种选择性剪接形式。 cDNA 与来自多种正常和肿瘤细胞和组织的 mRNA 杂交。使用 OGFr 反义寡核苷酸的功能研究表明细胞生长得到增强。荧光原位杂交（FISH）实验显示染色体位置为20q13.3。该 OGF 受体与经典阿片受体没有同源性。这些结果为 OGF 和 OGF 受体在调节人类生长过程中的相互作用提供了分子有效性。
The native opioid growth factor (OGF), [Met5]-enkephalin, is a tonic inhibitory peptide that modulates cell proliferation and tissue organization during development, cancer, cellular renewal, wound healing, and angiogenesis. OGF action is mediated by a receptor mechanism. We have cloned and sequenced cDNAs encoding multiple spliced forms of a human OGF receptor. The open reading frame in the longest cDNA was found to encode a protein of 697 amino acids, and 8 imperfect repeats of 20 amino acids each were a prominent feature. Altogether, five alternatively spliced forms were observed. The cDNA hybridized to mRNA from a variety of normal and neoplastic cells and tissues. Functional studies using antisense oligonucleotides to OGFr demonstrated an enhancement in cell growth. Fluorescent in situ hybridization (FISH) experiments showed the chromosomal location to be 20q13.3. This OGF receptor has no homology to classical opioid receptors. These results provide molecular validity for the interaction of OGF and OGF receptor in the regulation of growth processes in humans.