New Acid Biosensor for Taste Transduction Based on Extracellular Recording of PKD Channels
New Acid Biosensor for Taste Transduction Based on Extracellular Recording of PKD Channels
复制标题
DOI：
10.1109/jsen.2012.2210544
复制
发表时间：
2012-07
影响因子：
4.3
通讯作者：
Chunsheng Wu;L. Du;Liang Hu;Wei Zhang-;Luhang Zhao;Ping Wang
中科院分区：
文献类型：
--
作者：
Chunsheng Wu;L. Du;Liang Hu;Wei Zhang-;Luhang Zhao;Ping Wang
本文介绍了一种基于多囊性肾病（PKD）通道的生物传感器，用于研究味觉信号转导的细胞外微电极阵列（MEA）记录。PKD通道最近被提出作为酸味感觉的候选通道，在人胚胎肾-293细胞中异源表达，并与MEA芯片偶联作为敏感元件。MEA芯片可作为传感器，通过细胞外记录的方式，长期监测PKD通道对酸刺激的反应。结果表明，该生物传感器能够成功地记录PKD通道对酸刺激的特异性关闭反应。此外，该生物传感器可作为一种新的工具，PKD通道的功能测定。该传感器为味觉信号转导，特别是离子通道介导的味觉信号转导机制的研究提供了一种新的技术途径。此外，该生物传感器还具有开发成用于检测酸性物质的电子舌的潜力。
This paper describes a polycystic kidney disease-like (PKD) channel-based biosensor for the research of taste transduction by extracellular recording using a micro electrode array (MEA). PKD channels, which were recently proposed as candidates for sour sensation, are heterologously expressed in human embryo kidney-293 cells and coupled with MEA chips to serve as sensitive elements. MEA chips are used as transducers to monitor the responses of PKD channels to sour stimulations by extracellular recording in a noninvasive way for a long term. The results indicate that this biosensor can successfully record the special off-responses of PKD channels to sour stimulations. In addition, this biosensor can be used as a new tool for the functional assays of PKD channels. It is suggested that this biosensor may provide a promising alternative technique for the research of taste transduction, especially for the mechanisms of taste transduction mediated by ion channels. Furthermore, this biosensor also holds the potentials to be developed into electronic tongues for the detection of sour substances.