Discriminating nevus and melanoma on paraffin-embedded skin biopsies using FTIR micro spectroscopy
Discriminating nevus and melanoma on paraffin-embedded skin biopsies using FTIR micro spectroscopy
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DOI：
10.1016/j.bbagen.2005.04.020
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发表时间：
2005-08-05
影响因子：
3
通讯作者：
Manfait, M
中科院分区：
文献类型：
--
作者：
Tfayli, A;Piot, O;Manfait, M
FTIR显微光谱学与聚类分析相结合，已被用于表征皮肤组织，以区分癌变和非癌变组织。这项体外研究的主要目的是证明红外光谱成像在石蜡活检中区分色素痣（良性皮肤病变）和黑色素瘤（恶性皮肤病变）的适用性。对未脱蜡的痣和黑素瘤石蜡包埋样品进行红外光谱采集。尽管石蜡在这些光谱中有重要贡献，但仍有可能找到有意义和可区分的光谱区域。首先进行光谱成像以定位不同的皮肤层（真皮和表皮）。从图像中提取的光谱经过分层分类算法，使用与DNA和黑色素含量的振动相对应的选择光谱窗口，可以从痣中区分黑色素瘤。皮肤病变的多样性和皮肤的直接可及性使该器官成为使用该技术进行研究的有趣领域。(c) 2005 Elsevier B.V.版权所有
FTIR microspectroscopy, in combination with cluster analysis, has been used to characterise skin tissues, in order to discriminate cancerous from non-cancerous ones. The main objective of this in vitro study was to demonstrate the applicability of infrared spectral imaging to separate, on paraffinised biopsies, pigmented nevi (benign skin lesions) from melanomas (malignant skin lesions). Infrared spectra were collected from paraffin-embedded samples of nevi and melanomas, without deparaffinisation. Despite the important contribution of the paraffin in these spectra, it was possible to find meaningful and discriminating spectral regions. Spectral imaging was first performed to localize different skin layers (dermis and epidermis). Spectra extracted from the images were subjected to hierarchical classification algorithm, which allowed the discrimination of melanomas from the nevi, using selected spectral windows that correspond to vibrations of DNA and melanin content. The diversity of skin lesions and direct accessibility to the skin make this organ an interesting field of investigation using this technique. (c) 2005 Elsevier B.V. All rights reserved.