BCG stimulated dendritic cells induce an interleukin-10 producing T-cell population with no T helper 1 or T helper 2 bias in vitro
BCG stimulated dendritic cells induce an interleukin-10 producing T-cell population with no T helper 1 or T helper 2 bias in vitro
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DOI：
10.1111/j.1365-2567.2007.02575.x
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发表时间：
2007-06-01
期刊：
影响因子：
6.4
通讯作者：
Yazdanbakhsh, Maria
中科院分区：
文献类型：
--
作者：
Larsen, Jeppe Madura;Benn, Christine Stabell;Yazdanbakhsh, Maria
牛分枝杆菌卡介苗 (BCG) 疫苗对降低低收入国家儿童总体死亡率具有有益影响；这不能仅仅用预防结核病死亡来解释。卡介苗疫苗的有益作用可能是由于增强促炎机制、帮助新生儿抵抗感染，或者是诱导免疫调节网络限制明显炎症和严重病理。我们的目的是研究活卡介苗对树突状细胞 (DC) 极化 T 细胞反应能力的影响。单核细胞衍生的 DC 在存在或不存在 BCG 的情况下成熟。通过 CD83 表达、白细胞介素 12 (IL-12) 和 IL-10 产生以及极化 T 细胞反应的能力来评估 DC 表型。在用 CD40 配体刺激后，在 BCG 存在下成熟的 DC 显示出 IL-10 的增加和 IL-12 的产生减少。这些 DC 启动幼稚 T 细胞发育成产生 IL-10 的 T 细胞，不存在辅助性 T 1 或辅助性 T 2 偏好。这些结果表明，BCG 疫苗接种可能会导致产生 IL-10 的 DC 以及产生 IL-10 的 T 细胞的发育，这可能有助于限制暴露于病原体的婴儿的明显炎症，从而降低婴儿死亡率。
Mycobacterium bovis bacillus Calmette-Guerin (BCG) vaccine has been associated with beneficial effects on overall childhood mortality in low-income countries; this cannot be explained merely by the prevention of tuberculosis (TB) deaths. The beneficial effects of BCG vaccine could be the result of either strengthening of pro-inflammatory mechanisms, helping neonates to fight infections, or the induction of an immune-regulatory network restricting overt inflammation and intense pathology. We aimed to study the effect of live BCG on the ability of dendritic cells (DCs) to polarize T-cell responses. Monocyte-derived DCs were matured in the presence or absence of BCG. The DC phenotype was assessed by CD83 expression, interleukin-12 (IL-12) and IL-10 production, as well as for the ability to polarize T-cell responses. Following stimulation with CD40 ligand, DCs matured in the presence of BCG showed enhanced IL-10 and diminished IL-12 production. These DCs primed naive T cells to develop into IL-10-producing T cells, with no T helper 1 or T helper 2 bias. These results suggest that BCG vaccination might result in the development of IL-10-producing DCs as well as IL-10-producing T cells that could contribute to restricting overt inflammation in infants exposed to pathogens and thus lead to lower infant mortality.