KIN10 promotes stomatal development through stabilization of the SPEECHLESS transcription factor
KIN10 promotes stomatal development through stabilization of the SPEECHLESS transcription factor
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KIN10 通过稳定 SPEECHLESS 转录因子促进气孔发育
DOI：
10.1038/s41467-020-18048-w
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发表时间：
2020-08-25
影响因子：
16.6
通讯作者：
Bai, Ming-Yi
中科院分区：
文献类型：
--
作者：
Han, Chao;Liu, Yue;Bai, Ming-Yi
气孔是调节植物与大气之间气体交换的表皮结构。气孔的形成受到多种发育和环境信号的调节，但这些信号如何协调控制这一过程仍不清楚。在这里，我们表明，保守的能量传感器激酶SnRK1促进气孔发育短日照或在液体培养条件下。SnRK1的催化α亚基KIN10的突变导致气孔指数下降，而KIN10的过表达显著诱导气孔发育。KIN10显示细胞类型特异性亚细胞定位模式。核定位的KIN10蛋白在气孔谱系细胞中高度富集，以磷酸化和稳定气孔形成的主要调节因子SPEECHLESS，从而促进气孔发育。我们的工作确定了连接能量信号和气孔发育的模块链接，并揭示了SnRK1存在多种调节机制来调节气孔发育以应对不断变化的环境。
Stomata are epidermal structures that modulate gas exchanges between plants and the atmosphere. The formation of stomata is regulated by multiple developmental and environmental signals, but how these signals are coordinated to control this process remains unclear. Here, we showed that the conserved energy sensor kinase SnRK1 promotes stomatal development under short-day photoperiod or in liquid culture conditions. Mutation ofKIN10, the catalytic α-subunit of SnRK1, results in the decreased stomatal index; while overexpression ofKIN10significantly induces stomatal development. KIN10 displays the cell-type-specific subcellular location pattern. The nuclear-localized KIN10 proteins are highly enriched in the stomatal lineage cells to phosphorylate and stabilize SPEECHLESS, a master regulator of stomatal formation, thereby promoting stomatal development. Our work identifies a module links connecting the energy signaling and stomatal development and reveals that multiple regulatory mechanisms are in place for SnRK1 to modulate stomatal development in response to changing environments.