DECREASED LEVEL OF NERVE GROWTH-FACTOR (NGF) AND ITS MESSENGER-RNA IN THE AGED RAT-BRAIN
DECREASED LEVEL OF NERVE GROWTH-FACTOR (NGF) AND ITS MESSENGER-RNA IN THE AGED RAT-BRAIN
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DOI：
10.1016/0169-328x(87)90044-1
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发表时间：
1987-12-01
期刊：
影响因子：
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通讯作者：
OLSON, L
中科院分区：
文献类型：
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作者：
LARKFORS, L;EBENDAL, T;OLSON, L
营养因子如神经生长因子（NGF）被认为支持神经元的存活、分化和维持。最近的研究结果表明，在皮层和海马区产生的神经生长因子所需的胆碱能神经元在基底前脑的功能。用酶免疫分析法和RNA印迹杂交法研究了成年和老年大鼠基底前脑胆碱能神经元支配的脑区的神经生长因子（NGF）蛋白和神经生长因子（NGFmRNA）。与成年（6个月）相比，老年（28个月）Fischer 344大鼠海马中的NGF蛋白水平降低了40%，而大脑皮质中未观察到变化。此外，减少了50%的NGF mRNA被发现在老年前脑（大脑皮层，海马，基底前脑和下丘脑）的样品相比，成人。因此，神经生长因子的缺乏可能会导致基底前脑胆碱能神经元的丧失，这种丧失通常伴随着正常的衰老，并导致认知功能的改变。
Trophic factors such as nerve growth factor (NGF) are thought to support survival, differentiation and maintenance of neurons. Recent results indicate that NGF produced in cortical and hippocampal areas is required for the function of cholinergic neurons in the basal forebrain. With the use of enzyme immunoassay and RNA blot hybridization we studied the NGF protein and NGF mRNA, respectively, in regions of the brain innervated by basal forebrain cholinergic neurons in adult and aged rats. Levels of NGF protein were decreased by 40% in hippocampus of aged (28 months) Fischer 344 rats compared with adults (6 months), whereas no alterations were observed in cerebral cortex. Moreover, a reduction by 50% in the NGF mRNA was found in samples of the aged forebrain (cerebral cortex, hippocampus, basal forebrain and hypothalamus) compared to the adult. NGF deficiencies may thus account for the loss of cholinergic neurons in the basal forebrain generally found to accompany normal aging and resulting in altered cognitive functions.