Programmable protein-protein conjugation via DNA-based self-assembly.
Programmable protein-protein conjugation via DNA-based self-assembly.
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DOI：
10.1039/c2cc30618b
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发表时间：
2012-05
影响因子：
4.9
通讯作者：
J. Shimada;T. Maruyama;Momoko Kitaoka;H. Yoshinaga;K. Nakano;N. Kamiya;M. Goto
中科院分区：
文献类型：
--
作者：
J. Shimada;T. Maruyama;Momoko Kitaoka;H. Yoshinaga;K. Nakano;N. Kamiya;M. Goto
蛋白质分子被精确地排列在一个可设计的DNA支架上，使用DNA适体彼此靠近。通过添加化学交联剂，相邻的蛋白质分子有效地和共价地相互交联而不失去其活性。
Protein molecules were precisely arrayed on a designable DNA scaffold close to each other using a DNA aptamer. By adding a chemical cross-linker, the neighboring protein molecules were effectively and covalently cross-linked to each other without losing their activities.