Composition and evolution of the vertebrate and mammalian selenoproteomes.
Composition and evolution of the vertebrate and mammalian selenoproteomes.
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脊椎动物和哺乳动物硒蛋白质组的组成和进化
DOI：
10.1371/journal.pone.0033066
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gladyshev VN
中科院分区：
文献类型：
--
作者：
Mariotti M;Ridge PG;Zhang Y;Lobanov AV;Pringle TH;Guigo R;Hatfield DL;Gladyshev VN
背景：硒是哺乳动物体内必需的微量元素，因为它以硒半胱氨酸(Sec)的形式存在于蛋白质中。人类基因组编码25个含SEC的蛋白质基因，小鼠和大鼠基因组编码24个。方法/主要发现我们应用基因预测和系统发育重建的方法，并辅以基因结构、选择性剪接异构体、非翻译区、SECIS元件和假基因的分析，对44种已测序脊椎动物的硒蛋白质组进行了表征。我们总共检测到了45个硒蛋白亚家族。其中在哺乳动物中发现了28种，在硬骨鱼中发现了41种。我们定义了祖先脊椎动物(28个蛋白质)和哺乳动物(25个蛋白质)的硒蛋白质组，并描述了它们是如何通过基因复制(20个事件)、基因丢失(10个事件)和用半胱氨酸取代SEC(12个事件)而沿着谱系进化的。我们发现，在早期哺乳动物中进化出一个无内含子的硒磷酸合成酶2基因，并在功能上取代了胎盘哺乳动物中原来的多外显子基因，而这两个基因在有袋类动物中仍然存在。哺乳动物的硫氧还蛋白还原酶1和硫氧还蛋白谷胱甘肽还原酶是从含有谷氧还蛋白结构域的祖先进化而来的，至今仍存在于鱼类中。SelenoProtein V和GPx6分别在胎盘哺乳动物中由SelW和GPx3的重复进化而来，并且GPx6独立地多次丢失SEC。硬骨鱼的特征是几个硒蛋白家族(GPX1、GPX3、Gpx4、DIO3、MsrB1、Selj、Selo、Selt、SelU1和SelW2)的重复。最后，我们报告了几种硒蛋白的新亚型的鉴定，并描述了异常保守的硒蛋白假基因。结论/意义这一分析首次对脊椎动物和哺乳动物的硒蛋白质组进行了全面的调查，并描绘了它们沿谱系的进化。它还提供了关于这些硒蛋白及其形式的丰富信息。
Background Selenium is an essential trace element in mammals due to its presence in proteins in the form of selenocysteine (Sec). Human genome codes for 25 Sec-containing protein genes, and mouse and rat genomes for 24. Methodology/Principal Findings We characterized the selenoproteomes of 44 sequenced vertebrates by applying gene prediction and phylogenetic reconstruction methods, supplemented with the analyses of gene structures, alternative splicing isoforms, untranslated regions, SECIS elements, and pseudogenes. In total, we detected 45 selenoprotein subfamilies. 28 of them were found in mammals, and 41 in bony fishes. We define the ancestral vertebrate (28 proteins) and mammalian (25 proteins) selenoproteomes, and describe how they evolved along lineages through gene duplication (20 events), gene loss (10 events) and replacement of Sec with cysteine (12 events). We show that an intronless selenophosphate synthetase 2 gene evolved in early mammals and replaced functionally the original multiexon gene in placental mammals, whereas both genes remain in marsupials. Mammalian thioredoxin reductase 1 and thioredoxin-glutathione reductase evolved from an ancestral glutaredoxin-domain containing enzyme, still present in fish. Selenoprotein V and GPx6 evolved specifically in placental mammals from duplications of SelW and GPx3, respectively, and GPx6 lost Sec several times independently. Bony fishes were characterized by duplications of several selenoprotein families (GPx1, GPx3, GPx4, Dio3, MsrB1, SelJ, SelO, SelT, SelU1, and SelW2). Finally, we report identification of new isoforms for several selenoproteins and describe unusually conserved selenoprotein pseudogenes. Conclusions/Significance This analysis represents the first comprehensive survey of the vertebrate and mammal selenoproteomes, and depicts their evolution along lineages. It also provides a wealth of information on these selenoproteins and their forms.
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影响因子：
4.8
作者：
Fomenko, Dmitri E.;Novoselov, Sergey V.;Gladyshev, Vadim N.
通讯作者：
Gladyshev, Vadim N.
影响因子：
7.7
作者：
Castellano, S;Novoselov, SV;Guigó, R
通讯作者：
Guigó, R
影响因子：
4.8
作者：
Dammeyer, Pascal;Damdimopoulos, Anastasios E.;Arner, Elias S. J.
通讯作者：
Arner, Elias S. J.
影响因子：
5.3
作者：
Conrad, M;Jakupoglu, C;Brielmeier, M
通讯作者：
Brielmeier, M
影响因子：
4.8
作者：
Gerashchenko, Maxim V.;Su, Dan;Gladyshev, Vadim N.
通讯作者：
Gladyshev, Vadim N.