Dynamic changes of paraoxonase 1 activity towards paroxon and phenyl acetate during coronary artery surgery.
Dynamic changes of paraoxonase 1 activity towards paroxon and phenyl acetate during coronary artery surgery.
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DOI：
10.1186/s12872-017-0528-z
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发表时间：
2017-04-04
影响因子：
2.1
通讯作者：
Zapolski T
中科院分区：
文献类型：
--
作者：
Wysocka A;Cybulski M;Berbeć H;Wysokiński A;Stążka J;Daniluk J;Zapolski T
血清对氧磷酶1 （PON1）是一种与高密度脂蛋白（HDL）颗粒相关的酶，可抑制血清脂蛋白和细胞膜的氧化。PON1活性在动脉粥样硬化和炎症性疾病患者中较低。体外循环移植术中引起的全身炎症反应可能导致术后并发症的发生。本研究的目的是估计对氧磷酶1 （PON1）活性对对氧磷和乙酸苯酯在冠状动脉手术期间和之后的动态变化。26例接受冠状动脉旁路移植术（CABG）的冠心病患者被纳入研究。分别于术前、主动脉结块后、手术结束后、术后6、18、30、48 h采集静脉血。在含有1.0 mM对氧磷和1.0 mM CaCl2的50 mM甘氨酸/NaOH缓冲液（pH 10.5）中分光光度法测定对氧磷酶活性。在含有1 mM乙酸苯酯和1 mM CaCl2的20 mM TrisCl缓冲液（pH 8.0）中测定芳基酯酶活性。PON1对对氧磷和乙酸苯酯的活性在主动脉交叉结块后明显降低，术后直接升高。术前PON1对对氧磷的活性和实验第七期PON1对乙酸苯的活性与术后并发症的发生呈负相关。对氧磷酶1血浆活性在冠脉搭桥手术期间明显降低。
Serum paraoxonase 1 (PON1), an enzyme associated with high – density lipoproteins (HDL) particles, inhibits the oxidation of serum lipoproteins and cell membranes. PON1 activity is lower in patients with atherosclerosis and in inflammatory diseases. The systemic inflammatory response provoked during cardiopulmonary bypass grafting may contribute to the development of postoperative complications. The aim of the present study was to estimate the dynamic changes in paraoxonase 1 (PON1) activity towards paraoxon and phenyl acetate during and after coronary artery surgery. Twenty six patients with coronary heart disease undergoing coronary artery bypass grafting (CABG) were enrolled into the study. Venous blood samples were obtained preoperatively, after aortic clumping, after the end of operation, at 6, 18, 30 and 48 h after operation. Paraoxonase activity was measured spectrophotometrically in 50 mM glycine/NaOH buffer (pH 10.5) containing 1.0 mM paraoxon, and 1.0 mM CaCl2. Arylesterase activity was measured in 20 mM TrisCl buffer (pH 8.0) containing 1 mM phenyl acetate and 1 mM CaCl2. PON1 activity toward paraoxon and phenyl acetate significantly decreased after aorta cross clumping and increased directly after operation. PON1 activity towards paraoxon in preoperative period and PON1 activity towards phenyl acetate in seventh stage of experiment tended to inversely correlate with the occurrence of postoperative complications. The paraoxonase 1 plasma activity is markedly reduced during CABG surgery.