Immature and mature neurons coexist among glial scars after rat traumatic brain injury
Immature and mature neurons coexist among glial scars after rat traumatic brain injury
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DOI：
10.1179/016164107x208086
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发表时间：
2007-10-01
影响因子：
1.9
通讯作者：
Ito, Hiroyuki
中科院分区：
文献类型：
--
作者：
Itoh, Tatsuki;Satou, Takao;Ito, Hiroyuki
目的：脑损伤后受损区域周围的胶质细胞瘢痕抑制存活神经元突起的伸长和轴突的可塑性，从而阻止神经网络的再生。然而，脑损伤后神经干细胞在神经胶质瘢痕中的生成、分化和成熟的研究尚未见报道。方法：采用大鼠创伤性脑损伤模型，研究损伤区神经胶质细胞增生与新生神经元成熟的时间关系。结果：损伤后1~7天，损伤区周围可见大量巢蛋白阳性细胞。损伤后3天，许多小巢蛋白阳性细胞呈星形细胞形态。损伤后1~30d，损伤区周围可见双皮质醇(DCX)阳性细胞。伤后3天和7天，少量巢蛋白阳性细胞呈胶质纤维酸性蛋白(GFAP)阳性。损伤后7d，损伤灶内的胶质细胞增生中可见DCX阳性细胞。伤后30d，在胶质瘢痕附近和胶质瘢痕间可见DCX阳性细胞，其中有少量神经元呈NeuN阳性。有观点认为，促进脑损伤后胶质瘢痕附近和之间新形成的未成熟神经元的成熟和分化，可能改善脑损伤后胶质瘢痕所致的脑功能障碍。
Objectives: Glial scars around a damaged area after brain injury inhibit neurite elongation from surviving neurons and axonal plasticity, and thus prevent neural network regeneration. However, the generation, differentiation and maturation of neural stem cells (NSCs) among glial scars after brain injury have not yet been reported.Methods: In the present study, we investigated the chronological relationship between gliosis and maturation of new neurons around a damaged area using a rat traumatic brain injury (TBI) model.Results: Between 1 and 7 days after injury, many nestin-positive cells were observed around the damaged area. Three days after injury, many small nestin-positive cells showed an astrocytic morphology. Between 1 and 30 days after injury, doublecortin (DCX)-positive cells were present around the damaged area. Three and 7 days after injury, a small number of nestin-positive cells were immunopositive for glial fibrillary acidic protein (GFAP). Seven days after injury, there were DCX-positive cells in the gliosis occurring in the lesion. Thirty days after injury, DCX-positive cells were observed near and among the glial scars and a small number of these cells were immunopositive for NeuN.Discussion: These results suggest that DCX-positive cells were present near and among the glial scars after brain injury, and that these cells changed from immature to mature neurons. It is considered that promotion of the maturation and differentiation of newly formed immature neurons near and among glial scars after injury may improve the brain dysfunction induced by glial scars after brain injury.