Mutations in the essential spindle checkpoint gene bub1 cause chromosome missegregation and fail to block apoptosis in Drosophila.
Mutations in the essential spindle checkpoint gene bub1 cause chromosome missegregation and fail to block apoptosis in Drosophila.
复制标题
DOI：
10.1083/jcb.146.1.13
复制
发表时间：
1999-07-12
期刊：
影响因子：
--
通讯作者：
Goldberg ML
中科院分区：
文献类型：
--
作者：
Basu J;Bousbaa H;Logarinho E;Li Z;Williams BC;Lopes C;Sunkel CE;Goldberg ML
我们已经确定了果蝇有丝分裂检查点控制蛋白Bub 1的特征，并获得了Bub 1基因的突变。果蝇Bub 1强烈定位于尚未到达中期板的有丝分裂和减数分裂染色体的着丝粒/动粒。动物纯合的P-元素诱导的，近零突变的bub 1死亡在晚期幼虫/蛹阶段由于严重的有丝分裂异常，表明旁路的检查点功能。这些异常包括加速退出中期和染色体错误分离和断裂。染色体断裂可能导致突变体中细胞凋亡水平显著升高。我们还研究了Bub 1和其他动粒组分之间的关系。我们表明，Bub 1激酶活性是不需要的3F 3/2表位在前期/前中期磷酸化，但需要在中期3F 3/2去磷酸化。无论是3F 3/2去磷酸化还是Bub 1从动粒的丢失都不是进入后期的先决条件。Bub 1定位于动粒不依赖于基因zw 10、rod、波罗或fizzy的产物，这表明动粒是由几个独立的染色体构建的。
We have characterized the Drosophila mitotic checkpoint control protein Bub1 and obtained mutations in the bub1 gene. Drosophila Bub1 localizes strongly to the centromere/kinetochore of mitotic and meiotic chromosomes that have not yet reached the metaphase plate. Animals homozygous for P-element–induced, near-null mutations of bub1 die during late larval/pupal stages due to severe mitotic abnormalities indicative of a bypass of checkpoint function. These abnormalities include accelerated exit from metaphase and chromosome missegregation and fragmentation. Chromosome fragmentation possibly leads to the significantly elevated levels of apoptosis seen in mutants. We have also investigated the relationship between Bub1 and other kinetochore components. We show that Bub1 kinase activity is not required for phosphorylation of 3F3/2 epitopes at prophase/prometaphase, but is needed for 3F3/2 dephosphorylation at metaphase. Neither 3F3/2 dephosphorylation nor loss of Bub1 from the kinetochore is a prerequisite for anaphase entry. Bub1's localization to the kinetochore does not depend on the products of the genes zw10, rod, polo, or fizzy, indicating that the kinetochore is constructed from several independent subassemblies.
登录
查看更多内容
影响因子：
64.8
作者：
Cahill, DP;Lengauer, C;Vogelstein, B
通讯作者：
Vogelstein, B
影响因子：
5.3
作者：
Farr, KA;Hoyt, MA
通讯作者：
Hoyt, MA
DOI：
10.1159/000131438
发表时间：
1980-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
BRINKLEY, BR;COX, SM;PEPPER, DA
通讯作者：
PEPPER, DA
DOI：
10.1083/jcb.143.7.1775
发表时间：
1998-12-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bernard P;Hardwick K;Javerzat JP
通讯作者：
Javerzat JP
影响因子：
56.9
作者：
Elledge, SJ
通讯作者：
Elledge, SJ