Dynamic localization of RNase MRP RNA in the nucleolus observed by fluorescent RNA cytochemistry in living cells.
Dynamic localization of RNase MRP RNA in the nucleolus observed by fluorescent RNA cytochemistry in living cells.
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DOI：
10.1083/jcb.131.6.1649
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发表时间：
1995-12
期刊：
影响因子：
--
通讯作者：
Pederson T
中科院分区：
文献类型：
--
作者：
Jacobson MR;Cao LG;Wang YL;Pederson T
MRP RNA（核糖核蛋白酶RNase MRP的RNA组分）的动态核内定位通过荧光RNA细胞化学方法在活细胞中检测（Wang，J.，L- G.曹玉- L. Wang和T.佩德森1991. Proc. Natl. Acad. Sci. USA. 88：7391-7395）。正常大鼠肾（NRK）上皮细胞核显微注射后，MRP RNA迅速积聚在核仁中。定位是专门在致密的纤维组成部分的核仁中，所揭示的免疫细胞化学与单克隆抗体对fibrillarin，一个已知的致密的纤维组成蛋白，以及通过数字光学切片显微镜和3-D立体重建。当MRP RNA被注射到细胞质中时，它不会被输入到细胞核中。核显微注射的突变MRP RNA显示，核仁定位需要一个序列元件（核苷酸23-62）先前牵连作为一个结合位点的核仁蛋白质，抗原。这些结果证明了MRP RNA在细胞核中的动态定位，并为MRP RNA的核仁靶向提供了重要的见解。
The dynamic intra-nuclear localization of MRP RNA, the RNA component of the ribonucleoprotein enzyme RNase MRP, was examined in living cells by the method of fluorescent RNA cytochemistry (Wang, J., L.-G. Cao, Y.-L. Wang, and T. Pederson. 1991. Proc. Natl. Acad. Sci. USA. 88:7391-7395). MRP RNA very rapidly accumulated in nucleoli after nuclear microinjection of normal rat kidney (NRK) epithelial cells. Localization was specifically in the dense fibrillar component of the nucleolus, as revealed by immunocytochemistry with a monoclonal antibody against fibrillarin, a known dense fibrillar component protein, as well as by digital optical sectioning microscopy and 3-D stereo reconstruction. When MRP RNA was injected into the cytoplasm it was not imported into the nucleus. Nuclear microinjection of mutant MRP RNAs revealed that nucleolar localization requires a sequence element (nucleotides 23-62) previously implicated as a binding site for a nucleolar protein, the To antigen. These results demonstrate the dynamic localization of MRP RNA in the nucleus and provide important insights into the nucleolar targeting of MRP RNA.