Neurovascular coupling and bilateral connectivity during NREM and REM sleep.
Neurovascular coupling and bilateral connectivity during NREM and REM sleep.
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DOI：
10.7554/elife.62071
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发表时间：
2020-10-29
期刊：
影响因子：
7.7
通讯作者：
Drew PJ
中科院分区：
文献类型：
--
作者：
Turner KL;Gheres KW;Proctor EA;Drew PJ
为了了解唤醒状态如何影响脑血流动力学和神经血管耦合，我们监测了未麻醉、头部固定小鼠的神经活动、行为和血流动力学信号。小鼠在成像过程中经常睡着，这些睡眠事件与清醒期穿插。在NREM和REM睡眠期间，小鼠的脑血容量（[HbT]）和小动脉直径相对于清醒状态大幅增加，比感官刺激诱发的大2到5倍。在NREM期间，[HbT]的双侧低频振荡幅度显著增加，神经活动与血流动力学信号之间的一致性高于清醒静息和REM状态。神经活动和[HbT]的双边相关性在NREM期间最高，在清醒状态下最低。皮层中的血流动力学信号受到唤醒状态的强烈调制，并且睡眠期间的变化远大于感觉诱发的反应。
To understand how arousal state impacts cerebral hemodynamics and neurovascular coupling, we monitored neural activity, behavior, and hemodynamic signals in un-anesthetized, head-fixed mice. Mice frequently fell asleep during imaging, and these sleep events were interspersed with periods of wake. During both NREM and REM sleep, mice showed large increases in cerebral blood volume ([HbT]) and arteriole diameter relative to the awake state, two to five times larger than those evoked by sensory stimulation. During NREM, the amplitude of bilateral low-frequency oscillations in [HbT] increased markedly, and coherency between neural activity and hemodynamic signals was higher than the awake resting and REM states. Bilateral correlations in neural activity and [HbT] were highest during NREM, and lowest in the awake state. Hemodynamic signals in the cortex are strongly modulated by arousal state, and changes during sleep are substantially larger than sensory-evoked responses.