Sequence, regulation, and evolution of the maize 22-kD α zein in gene family
Sequence, regulation, and evolution of the maize 22-kD α zein in gene family
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DOI：
10.1101/gr.197301
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发表时间：
2001-11-01
期刊：
影响因子：
7
通讯作者：
Messing, J
中科院分区：
文献类型：
--
作者：
Song, RT;Llaca, V;Messing, J
我们已经分离并测序了玉米22-kD α玉米醇溶蛋白（zlC）基因家族的所有23个成员。这是迄今为止从单个基因组中测序的最大的植物基因家族之一。圆的，包括最大的连续基因组DNA玉米与346，292 bp的日期。22个zlC成员在染色体4S上的大致串联阵列中被发现，形成168，489-bp长的密集基因簇。该基因家族的第23个拷贝也位于染色体4S上靠近着丝粒20 cM的类似位点，似乎是floury-2（fl 2）突变的野生型等位基因。基于对玉米cDNA数据库的分析，这些基因中只有七个似乎被表达，包括f12等位基因。簇中的表达基因散布着非表达基因。有趣的是，一些表达的基因在转录调控上不同。基因扩增出现在基因块中，解释了玉米进化过程中簇的快速而紧凑的扩张。
We have isolated and sequenced all 23 members of the 22-kD alpha zein (zlC) gene family of maize. This is one of the largest plant gene families that has been sequenced from a single genetic back.-round and includes the largest contiguous genomic DNA from maize with 346,292 bp to date. Twenty-two of the zlC members are found in a roughly tandem array on chromosome 4S Forming a dense gene cluster 168,489-bp long. The twenty-third copy of the gene family is also located on chromosome 4S at a site similar to 20 cM closer to the centromere and appears to be the wild-type allele of the floury-2 (fl2) mutation. On the basis of an analysis of maize cDNA databases, only seven of these genes appear to be expressed including the fl2 allele. The expressed genes in the cluster are interspersed with nonexpressed genes. Interestingly, some of the expressed genes differ in their transcriptional regulation. Gene amplification appears to be in blocks of genes explaining the rapid and compact expansion of the cluster during the evolution of maize.