Sorting by diffusion: An asymmetric obstacle course for continuous molecular separation
Sorting by diffusion: An asymmetric obstacle course for continuous molecular separation
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DOI：
10.1073/pnas.96.24.13762
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发表时间：
1999-11-23
影响因子：
11.1
通讯作者：
Austin, RH
中科院分区：
文献类型：
--
作者：
Chou, CF;Bakajin, O;Austin, RH
通过观察不同大小的DNA分子的运动，已经证明了采用微加工筛的分离技术。该筛由障碍物的二维晶格组成，其不对称的布置纠正了被驱动通过该装置的分子的布朗运动，使它们遵循取决于其扩散系数的路径。在4英寸（10-cm）的硅晶片中可以实现尺寸范围为15-30 kbp的DNA分子长度的标称6%分辨率。这种方法的优点是可以连续加载和分选样品，与凝胶电泳中常用的分批模式相反。
A separation technique employing a microfabricated sieve has been demonstrated by observing the motion of DNA molecules of different size. The sieve consists of a two-dimensional lattice of obstacles whose asymmetric disposition rectifies the Brownian motion of molecules driven through the device, causing them to follow paths that depend on their diffusion coefficient. A nominal 6% resolution by length of DNA molecules in the size range 15-30 kbp may be achieved in a 4-inch (10-cm) silicon wafer. The advantage of this method is that samples can be loaded and sorted continuously, in contrast to the batch mode commonly used in gel electrophoresis.