TECHNIQUES FOR MEASURING BLOOD-FLOW IN SPLANCHNIC TISSUES OF CATTLE
TECHNIQUES FOR MEASURING BLOOD-FLOW IN SPLANCHNIC TISSUES OF CATTLE
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DOI：
10.3168/jds.s0022-0302(89)79270-5
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发表时间：
1989-06-01
影响因子：
3.5
通讯作者：
STROUD, BH
中科院分区：
文献类型：
--
作者：
HUNTINGTON, GB;REYNOLDS, CK;STROUD, BH
研究营养吸收和代谢的一个有用的方法是在体内测量通过门静脉引流的内脏和肝脏的血流量以及血液代谢物的通量，该方法的成功应用需要在适当的血管中正确放置慢性导管。此外，导管必须保持足够长的时间，以允许动物从手术中恢复并完成实验方案。本文介绍了外科技术，安装慢性导管肠系膜静脉，肝门静脉，肝静脉牛。还描述了用于进入动脉血液的技术。描述了手术、血液取样和血流测定所需的材料、设备和用品。建议采用商业供应来源。通过对氨基马尿酸盐的下游稀释来测量血流量，将对氨基马尿酸盐注入肠系膜静脉。提供了三种类型牛的血流数据示例。
A useful approach of the study of nutrient absorption and metabolism is in vivo measurement of blood flow across portal-drained viscera and liver, and flux of bloodborne metabolites, successful application of the approach requires correct placement of chronic catheters in appropriate blood vessels. Additionally, catheters must stay patent long enough to allow the animal to recover from surgery and to complete an experimental protocol. This paper describes surgical techniques to install chronic catheters in mesenteric veins, the hepatic portal vein, and an hepatic vein of cattle. Techniques for access to arterial blood are described also. Materials, equipment, and supplies frequired for surgery, blood sampling, and blood flow determination are described. Commercial sources of supplies are suggested. Blood flow is measured by downstream dilution of para-aminohippurate, which is infued into a mesenteric vein. Examples of blood flow data for three types of cattle are provided.